336d: Difference between revisions

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==Overview==

The DNA fragment, d(CG)3, was co-crystallized with, N-(3-amino-propyl)-N-(5-aminopropyl)-l,4 -diaminobutane (thermospermine;, PA(334)), a polyamine metabolized from the nucleic acid. By using a good, crystal with dimensions of 0.5 x 0.5 x 0.5 mm3, X-ray intensity data were, collected up to 1.0 A resolution. Two thermospermine molecules and a, magnesium cation were bound to the left-handed double-helical d(CG)3, molecule. The d(CG)3 molecule adopted the left-handed Z-conformation and, two thermospermine molecules and a magnesium cation neutralized the, negative charges of the phosphate groups of the d(CG)3 molecule., Furthermore, the binding modes between d(CG)3 and thermospermine were, different from those of d(CG)3 complexes with PA(24), spermidine and, spermine. This is the first case in which it was determined by X-ray, crystallographic analysis that one of two thermospermine molecules bound, three d(CG)3 duplexes which were symmetrically related to each other, and, the other formed two hydrogen bonds at the N(5) and N(9) atoms with two, adjacent nucleotide phosphate groups of a single d(CG)3 strand at the, minor groove. Furthermore, no direct coordination bond was found between, the d(CG)3 molecule and the magnesium cation.

The DNA fragment, d(CG)3, was co-crystallized with N-(3-amino-propyl)-N-(5-aminopropyl)-l,4 -diaminobutane (thermospermine; PA(334)), a polyamine metabolized from the nucleic acid. By using a good crystal with dimensions of 0.5 x 0.5 x 0.5 mm3, X-ray intensity data were collected up to 1.0 A resolution. Two thermospermine molecules and a magnesium cation were bound to the left-handed double-helical d(CG)3 molecule. The d(CG)3 molecule adopted the left-handed Z-conformation and two thermospermine molecules and a magnesium cation neutralized the negative charges of the phosphate groups of the d(CG)3 molecule. Furthermore, the binding modes between d(CG)3 and thermospermine were different from those of d(CG)3 complexes with PA(24), spermidine and spermine. This is the first case in which it was determined by X-ray crystallographic analysis that one of two thermospermine molecules bound three d(CG)3 duplexes which were symmetrically related to each other, and the other formed two hydrogen bonds at the N(5) and N(9) atoms with two adjacent nucleotide phosphate groups of a single d(CG)3 strand at the minor groove. Furthermore, no direct coordination bond was found between the d(CG)3 molecule and the magnesium cation.

==About this Structure==

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Interaction between left-handed Z-DNA and polyamine - 3. The crystal structure of the d(CG)3 and thermospermine complex., Ohishi H, Terasoma N, Nakanishi I, van der Marel G, van Boom JH, Rich A, Wang AH, Hakoshima T, Tomita K, FEBS Lett. 1996 Dec 2;398(2-3):291-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8977125 8977125]

[[Category: Protein complex]]

[[Category: Boom, J.H.Van.]]

[[Category: Boom, J H.Van.]]

[[Category: Hakoshima, T.]]

[[Category: Marel, G.Van.Der.]]

[[Category: Marel, G Van Der.]]

[[Category: Nakanishi, I.]]

[[Category: Ohishi, H.]]

[[Category: Rich, A.]]

[[Category: Terasoma, N.]]

[[Category: Tomita, K.I.]]

[[Category: Tomita, K I.]]

[[Category: Wang, A.H.J.]]

[[Category: Wang, A H.J.]]

[[Category: MG]]

[[Category: TER]]

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[[Category: z-dna]]

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@@ Line 4: / Line 4: @@
 ==Overview==
-The DNA fragment, d(CG)3, was co-crystallized with, N-(3-amino-propyl)-N-(5-aminopropyl)-l,4 -diaminobutane (thermospermine;, PA(334)), a polyamine metabolized from the nucleic acid. By using a good, crystal with dimensions of 0.5 x 0.5 x 0.5 mm3, X-ray intensity data were, collected up to 1.0 A resolution. Two thermospermine molecules and a, magnesium cation were bound to the left-handed double-helical d(CG)3, molecule. The d(CG)3 molecule adopted the left-handed Z-conformation and, two thermospermine molecules and a magnesium cation neutralized the, negative charges of the phosphate groups of the d(CG)3 molecule., Furthermore, the binding modes between d(CG)3 and thermospermine were, different from those of d(CG)3 complexes with PA(24), spermidine and, spermine. This is the first case in which it was determined by X-ray, crystallographic analysis that one of two thermospermine molecules bound, three d(CG)3 duplexes which were symmetrically related to each other, and, the other formed two hydrogen bonds at the N(5) and N(9) atoms with two, adjacent nucleotide phosphate groups of a single d(CG)3 strand at the, minor groove. Furthermore, no direct coordination bond was found between, the d(CG)3 molecule and the magnesium cation.
+The DNA fragment, d(CG)3, was co-crystallized with N-(3-amino-propyl)-N-(5-aminopropyl)-l,4 -diaminobutane (thermospermine; PA(334)), a polyamine metabolized from the nucleic acid. By using a good crystal with dimensions of 0.5 x 0.5 x 0.5 mm3, X-ray intensity data were collected up to 1.0 A resolution. Two thermospermine molecules and a magnesium cation were bound to the left-handed double-helical d(CG)3 molecule. The d(CG)3 molecule adopted the left-handed Z-conformation and two thermospermine molecules and a magnesium cation neutralized the negative charges of the phosphate groups of the d(CG)3 molecule. Furthermore, the binding modes between d(CG)3 and thermospermine were different from those of d(CG)3 complexes with PA(24), spermidine and spermine. This is the first case in which it was determined by X-ray crystallographic analysis that one of two thermospermine molecules bound three d(CG)3 duplexes which were symmetrically related to each other, and the other formed two hydrogen bonds at the N(5) and N(9) atoms with two adjacent nucleotide phosphate groups of a single d(CG)3 strand at the minor groove. Furthermore, no direct coordination bond was found between the d(CG)3 molecule and the magnesium cation.
 ==About this Structure==
@@ Line 12: / Line 12: @@
 Interaction between left-handed Z-DNA and polyamine - 3. The crystal structure of the d(CG)3 and thermospermine complex., Ohishi H, Terasoma N, Nakanishi I, van der Marel G, van Boom JH, Rich A, Wang AH, Hakoshima T, Tomita K, FEBS Lett. 1996 Dec 2;398(2-3):291-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8977125 8977125]
 [[Category: Protein complex]]
-[[Category: Boom, J.H.Van.]]
+[[Category: Boom, J H.Van.]]
 [[Category: Hakoshima, T.]]
-[[Category: Marel, G.Van.Der.]]
+[[Category: Marel, G Van Der.]]
 [[Category: Nakanishi, I.]]
 [[Category: Ohishi, H.]]
 [[Category: Rich, A.]]
 [[Category: Terasoma, N.]]
-[[Category: Tomita, K.I.]]
+[[Category: Tomita, K I.]]
-[[Category: Wang, A.H.J.]]
+[[Category: Wang, A H.J.]]
 [[Category: MG]]
 [[Category: TER]]
@@ Line 26: / Line 26: @@
 [[Category: z-dna]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 21:35:47 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:02:10 2008''