377d: Difference between revisions

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==Overview==

The crystal structure of the alternating RNA r(CGUAC)dG with a 3'-terminal, deoxy G residue has been determined at 1.76 A resolution. The crystal, belongs to the orthorhombic space group C2221, unit-cell dimensions a =, 29.53, b = 44.61 and c = 94.18 A, with two independent duplexes (I and II), per asymmetric unit. The structure was solved by the molecular-replacement, method. The final R factor was 18.8% using 4757 reflections in the, resolution range 8.0-1.76 A. The model contains a total of 496 atoms and, 85 solvent molecules. The two duplexes form the repeating unit and stack, in the usual head-to-tail (5',3'/5',3') fashion into a pseudocontinuous, helical column. Almost all of the 2'-hydroxyl groups are engaged in the, three modes of water-mediated interactions to the base N3/O2 atoms, the, sugar O4' atoms and the backbone phosphates. Thus, the 2'-hydroxyl group, of RNA is probably contributing to the stability of the duplexes.

The crystal structure of the alternating RNA r(CGUAC)dG with a 3'-terminal deoxy G residue has been determined at 1.76 A resolution. The crystal belongs to the orthorhombic space group C2221, unit-cell dimensions a = 29.53, b = 44.61 and c = 94.18 A, with two independent duplexes (I and II) per asymmetric unit. The structure was solved by the molecular-replacement method. The final R factor was 18.8% using 4757 reflections in the resolution range 8.0-1.76 A. The model contains a total of 496 atoms and 85 solvent molecules. The two duplexes form the repeating unit and stack in the usual head-to-tail (5',3'/5',3') fashion into a pseudocontinuous helical column. Almost all of the 2'-hydroxyl groups are engaged in the three modes of water-mediated interactions to the base N3/O2 atoms, the sugar O4' atoms and the backbone phosphates. Thus, the 2'-hydroxyl group of RNA is probably contributing to the stability of the duplexes.

==About this Structure==

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[[Category: Protein complex]]

[[Category: Biswas, R.]]

[[Category: Mitra, S.N.]]

[[Category: Mitra, S N.]]

[[Category: Sundaralingam, M.]]

[[Category: a-rna]]

[[Category: double helix]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:02:28 2008''

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@@ Line 4: / Line 4: @@
 ==Overview==
-The crystal structure of the alternating RNA r(CGUAC)dG with a 3'-terminal, deoxy G residue has been determined at 1.76 A resolution. The crystal, belongs to the orthorhombic space group C2221, unit-cell dimensions a =, 29.53, b = 44.61 and c = 94.18 A, with two independent duplexes (I and II), per asymmetric unit. The structure was solved by the molecular-replacement, method. The final R factor was 18.8% using 4757 reflections in the, resolution range 8.0-1.76 A. The model contains a total of 496 atoms and, 85 solvent molecules. The two duplexes form the repeating unit and stack, in the usual head-to-tail (5',3'/5',3') fashion into a pseudocontinuous, helical column. Almost all of the 2'-hydroxyl groups are engaged in the, three modes of water-mediated interactions to the base N3/O2 atoms, the, sugar O4' atoms and the backbone phosphates. Thus, the 2'-hydroxyl group, of RNA is probably contributing to the stability of the duplexes.
+The crystal structure of the alternating RNA r(CGUAC)dG with a 3'-terminal deoxy G residue has been determined at 1.76 A resolution. The crystal belongs to the orthorhombic space group C2221, unit-cell dimensions a = 29.53, b = 44.61 and c = 94.18 A, with two independent duplexes (I and II) per asymmetric unit. The structure was solved by the molecular-replacement method. The final R factor was 18.8% using 4757 reflections in the resolution range 8.0-1.76 A. The model contains a total of 496 atoms and 85 solvent molecules. The two duplexes form the repeating unit and stack in the usual head-to-tail (5',3'/5',3') fashion into a pseudocontinuous helical column. Almost all of the 2'-hydroxyl groups are engaged in the three modes of water-mediated interactions to the base N3/O2 atoms, the sugar O4' atoms and the backbone phosphates. Thus, the 2'-hydroxyl group of RNA is probably contributing to the stability of the duplexes.
 ==About this Structure==
@@ Line 13: / Line 13: @@
 [[Category: Protein complex]]
 [[Category: Biswas, R.]]
-[[Category: Mitra, S.N.]]
+[[Category: Mitra, S N.]]
 [[Category: Sundaralingam, M.]]
 [[Category: a-rna]]
 [[Category: double helix]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 21:37:11 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:02:28 2008''