417d: Difference between revisions

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==Overview==

The DNA hexamer d(CACGPG), in which dP is the ambivalent pyrimidine, nucleoside analogue 2'-deoxy-beta-d-ribofuranosyl-(6H,8H-3, 4-dihydropyrimido[4,5-c][1,2]oxazin-7-one), crystallises as a left-handed, Z-DNA duplex. X-ray analysis at 1.5 A shows that both P. A base-pairs are, of the wobble type. This result appears inconsistent with other evidence, from hybridisation and NMR studies of P-containing oligonucleotides, which, suggests that, while P can form stable base-pairs with either A or G, thymine-like properties are more pronounced. Thermal denaturation, experiments over a range of solution pH values indicate that protonation, of the P.A base-pairs is unlikely to be responsible for the anomalous, behaviour. No specific crystal packing effects can be identfied as an, explanation, and it is concluded that base stacking and other interactions, between nucleotide residues in Z-DNA are responsible.

The DNA hexamer d(CACGPG), in which dP is the ambivalent pyrimidine nucleoside analogue 2'-deoxy-beta-d-ribofuranosyl-(6H,8H-3, 4-dihydropyrimido[4,5-c][1,2]oxazin-7-one), crystallises as a left-handed Z-DNA duplex. X-ray analysis at 1.5 A shows that both P. A base-pairs are of the wobble type. This result appears inconsistent with other evidence from hybridisation and NMR studies of P-containing oligonucleotides, which suggests that, while P can form stable base-pairs with either A or G, thymine-like properties are more pronounced. Thermal denaturation experiments over a range of solution pH values indicate that protonation of the P.A base-pairs is unlikely to be responsible for the anomalous behaviour. No specific crystal packing effects can be identfied as an explanation, and it is concluded that base stacking and other interactions between nucleotide residues in Z-DNA are responsible.

==About this Structure==

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A thymine-like base analogue forms wobble pairs with adenine in a Z-DNA duplex., Schuerman GS, Van Meervelt L, Loakes D, Brown DM, Kong Thoo Lin P, Moore MH, Salisbury SA, J Mol Biol. 1998 Oct 9;282(5):1005-11. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9753550 9753550]

[[Category: Protein complex]]

[[Category: Brown, D.M.]]

[[Category: Brown, D M.]]

[[Category: Lin, P.K.T.]]

[[Category: Lin, P K.T.]]

[[Category: Loakes, D.]]

[[Category: Meervelt, L.Van.]]

[[Category: Meervelt, L Van.]]

[[Category: Moore, G.S.]]

[[Category: Moore, G S.]]

[[Category: Moore, M.H.]]

[[Category: Moore, M H.]]

[[Category: Schuerman, M.H.]]

[[Category: Schuerman, M H.]]

[[Category: p.a base-pairs]]

[[Category: p a base-pairs]]

[[Category: pyrimidine analogue]]

[[Category: z-dna]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:12:04 2008''

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@@ Line 4: / Line 4: @@
 ==Overview==
-The DNA hexamer d(CACGPG), in which dP is the ambivalent pyrimidine, nucleoside analogue 2'-deoxy-beta-d-ribofuranosyl-(6H,8H-3, 4-dihydropyrimido[4,5-c][1,2]oxazin-7-one), crystallises as a left-handed, Z-DNA duplex. X-ray analysis at 1.5 A shows that both P. A base-pairs are, of the wobble type. This result appears inconsistent with other evidence, from hybridisation and NMR studies of P-containing oligonucleotides, which, suggests that, while P can form stable base-pairs with either A or G, thymine-like properties are more pronounced. Thermal denaturation, experiments over a range of solution pH values indicate that protonation, of the P.A base-pairs is unlikely to be responsible for the anomalous, behaviour. No specific crystal packing effects can be identfied as an, explanation, and it is concluded that base stacking and other interactions, between nucleotide residues in Z-DNA are responsible.
+The DNA hexamer d(CACGPG), in which dP is the ambivalent pyrimidine nucleoside analogue 2'-deoxy-beta-d-ribofuranosyl-(6H,8H-3, 4-dihydropyrimido[4,5-c][1,2]oxazin-7-one), crystallises as a left-handed Z-DNA duplex. X-ray analysis at 1.5 A shows that both P. A base-pairs are of the wobble type. This result appears inconsistent with other evidence from hybridisation and NMR studies of P-containing oligonucleotides, which suggests that, while P can form stable base-pairs with either A or G, thymine-like properties are more pronounced. Thermal denaturation experiments over a range of solution pH values indicate that protonation of the P.A base-pairs is unlikely to be responsible for the anomalous behaviour. No specific crystal packing effects can be identfied as an explanation, and it is concluded that base stacking and other interactions between nucleotide residues in Z-DNA are responsible.
 ==About this Structure==
@@ Line 12: / Line 12: @@
 A thymine-like base analogue forms wobble pairs with adenine in a Z-DNA duplex., Schuerman GS, Van Meervelt L, Loakes D, Brown DM, Kong Thoo Lin P, Moore MH, Salisbury SA, J Mol Biol. 1998 Oct 9;282(5):1005-11. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9753550 9753550]
 [[Category: Protein complex]]
-[[Category: Brown, D.M.]]
+[[Category: Brown, D M.]]
-[[Category: Lin, P.K.T.]]
+[[Category: Lin, P K.T.]]
 [[Category: Loakes, D.]]
-[[Category: Meervelt, L.Van.]]
+[[Category: Meervelt, L Van.]]
-[[Category: Moore, G.S.]]
+[[Category: Moore, G S.]]
-[[Category: Moore, M.H.]]
+[[Category: Moore, M H.]]
-[[Category: Schuerman, M.H.]]
+[[Category: Schuerman, M H.]]
-[[Category: p.a base-pairs]]
+[[Category: p a base-pairs]]
 [[Category: pyrimidine analogue]]
 [[Category: z-dna]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 21:42:38 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:12:04 2008''