422d: Difference between revisions

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==Overview==

The crystal structure of an RNA dodecamer, r(GAUCACUUCGGU), was solved at, 2.6 A resolution by the molecular-replacement method and refined to an, R(work) of 18.8% (R(free) = 22.8%) using 2494 reflections. The dodecamer, crystallized in the monoclinic space group C2, with unit-cell parameters a, = 71.34, b = 39.98, c = 32.47 A, beta = 104.7 degrees and two independent, strands in the asymmetric unit. The dodecamer adopts an octamer duplex, structure with four 5'-overhang residues (G1A2U3C4), which form, Watson-Crick base pairs with another four 5'-overhang residues of a, symmetry-related duplex. The octamer duplex (ACUUCGGU) contains at its, center four mismatched base pairs flanked by two Watson-Crick base pairs., The mismatched bases form two G.U wobble base pairs at the ends and two, U.C base pairs at the center, with one base-base hydrogen bond N4(C).O4(U), and a water bridge connecting the N(3) of the cytosine and uridine. The, present study reinforces the concept of the stability of the conformation, of UUCG in RNA double-helical structures.

The crystal structure of an RNA dodecamer, r(GAUCACUUCGGU), was solved at 2.6 A resolution by the molecular-replacement method and refined to an R(work) of 18.8% (R(free) = 22.8%) using 2494 reflections. The dodecamer crystallized in the monoclinic space group C2, with unit-cell parameters a = 71.34, b = 39.98, c = 32.47 A, beta = 104.7 degrees and two independent strands in the asymmetric unit. The dodecamer adopts an octamer duplex structure with four 5'-overhang residues (G1A2U3C4), which form Watson-Crick base pairs with another four 5'-overhang residues of a symmetry-related duplex. The octamer duplex (ACUUCGGU) contains at its center four mismatched base pairs flanked by two Watson-Crick base pairs. The mismatched bases form two G.U wobble base pairs at the ends and two U.C base pairs at the center, with one base-base hydrogen bond N4(C).O4(U) and a water bridge connecting the N(3) of the cytosine and uridine. The present study reinforces the concept of the stability of the conformation of UUCG in RNA double-helical structures.

==About this Structure==

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[[Category: Protein complex]]

[[Category: Eswaramoorthy, S.]]

[[Category: Rao, S.T.]]

[[Category: Rao, S T.]]

[[Category: Sundaralingam, M.]]

[[Category: double helix]]

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@@ Line 4: / Line 4: @@
 ==Overview==
-The crystal structure of an RNA dodecamer, r(GAUCACUUCGGU), was solved at, 2.6 A resolution by the molecular-replacement method and refined to an, R(work) of 18.8% (R(free) = 22.8%) using 2494 reflections. The dodecamer, crystallized in the monoclinic space group C2, with unit-cell parameters a, = 71.34, b = 39.98, c = 32.47 A, beta = 104.7 degrees and two independent, strands in the asymmetric unit. The dodecamer adopts an octamer duplex, structure with four 5'-overhang residues (G1A2U3C4), which form, Watson-Crick base pairs with another four 5'-overhang residues of a, symmetry-related duplex. The octamer duplex (ACUUCGGU) contains at its, center four mismatched base pairs flanked by two Watson-Crick base pairs., The mismatched bases form two G.U wobble base pairs at the ends and two, U.C base pairs at the center, with one base-base hydrogen bond N4(C).O4(U), and a water bridge connecting the N(3) of the cytosine and uridine. The, present study reinforces the concept of the stability of the conformation, of UUCG in RNA double-helical structures.
+The crystal structure of an RNA dodecamer, r(GAUCACUUCGGU), was solved at 2.6 A resolution by the molecular-replacement method and refined to an R(work) of 18.8% (R(free) = 22.8%) using 2494 reflections. The dodecamer crystallized in the monoclinic space group C2, with unit-cell parameters a = 71.34, b = 39.98, c = 32.47 A, beta = 104.7 degrees and two independent strands in the asymmetric unit. The dodecamer adopts an octamer duplex structure with four 5'-overhang residues (G1A2U3C4), which form Watson-Crick base pairs with another four 5'-overhang residues of a symmetry-related duplex. The octamer duplex (ACUUCGGU) contains at its center four mismatched base pairs flanked by two Watson-Crick base pairs. The mismatched bases form two G.U wobble base pairs at the ends and two U.C base pairs at the center, with one base-base hydrogen bond N4(C).O4(U) and a water bridge connecting the N(3) of the cytosine and uridine. The present study reinforces the concept of the stability of the conformation of UUCG in RNA double-helical structures.
 ==About this Structure==
@@ Line 13: / Line 13: @@
 [[Category: Protein complex]]
 [[Category: Eswaramoorthy, S.]]
-[[Category: Rao, S.T.]]
+[[Category: Rao, S T.]]
 [[Category: Sundaralingam, M.]]
 [[Category: double helix]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 21:42:51 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:12:03 2008''