437d: Difference between revisions

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==Overview==

Many viruses regulate translation of polycistronic mRNA using a -1, ribosomal frameshift induced by an RNA pseudoknot. A pseudoknot has two, stems that form a quasi-continuous helix and two connecting loops. A 1.6 A, crystal structure of the beet western yellow virus (BWYV) pseudoknot, reveals rotation and a bend at the junction of the two stems. A loop base, is inserted in the major groove of one stem with quadruple-base, interactions. The second loop forms a new minor-groove triplex motif with, the other stem, involving 2'-OH and triple-base interactions, as well as, sodium ion coordination. Overall, the number of hydrogen bonds stabilizing, the tertiary interactions exceeds the number involved in Watson-Crick base, pairs. This structure will aid mechanistic analyses of ribosomal, frameshifting.

Many viruses regulate translation of polycistronic mRNA using a -1 ribosomal frameshift induced by an RNA pseudoknot. A pseudoknot has two stems that form a quasi-continuous helix and two connecting loops. A 1.6 A crystal structure of the beet western yellow virus (BWYV) pseudoknot reveals rotation and a bend at the junction of the two stems. A loop base is inserted in the major groove of one stem with quadruple-base interactions. The second loop forms a new minor-groove triplex motif with the other stem, involving 2'-OH and triple-base interactions, as well as sodium ion coordination. Overall, the number of hydrogen bonds stabilizing the tertiary interactions exceeds the number involved in Watson-Crick base pairs. This structure will aid mechanistic analyses of ribosomal frameshifting.

==About this Structure==

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Minor groove RNA triplex in the crystal structure of a ribosomal frameshifting viral pseudoknot., Su L, Chen L, Egli M, Berger JM, Rich A, Nat Struct Biol. 1999 Mar;6(3):285-92. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10074948 10074948]

[[Category: Protein complex]]

[[Category: Berger, J.M.]]

[[Category: Berger, J M.]]

[[Category: Chen, L.]]

[[Category: Egli, M.]]

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[[Category: viral frameshifting]]

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@@ Line 4: / Line 4: @@
 ==Overview==
-Many viruses regulate translation of polycistronic mRNA using a -1, ribosomal frameshift induced by an RNA pseudoknot. A pseudoknot has two, stems that form a quasi-continuous helix and two connecting loops. A 1.6 A, crystal structure of the beet western yellow virus (BWYV) pseudoknot, reveals rotation and a bend at the junction of the two stems. A loop base, is inserted in the major groove of one stem with quadruple-base, interactions. The second loop forms a new minor-groove triplex motif with, the other stem, involving 2'-OH and triple-base interactions, as well as, sodium ion coordination. Overall, the number of hydrogen bonds stabilizing, the tertiary interactions exceeds the number involved in Watson-Crick base, pairs. This structure will aid mechanistic analyses of ribosomal, frameshifting.
+Many viruses regulate translation of polycistronic mRNA using a -1 ribosomal frameshift induced by an RNA pseudoknot. A pseudoknot has two stems that form a quasi-continuous helix and two connecting loops. A 1.6 A crystal structure of the beet western yellow virus (BWYV) pseudoknot reveals rotation and a bend at the junction of the two stems. A loop base is inserted in the major groove of one stem with quadruple-base interactions. The second loop forms a new minor-groove triplex motif with the other stem, involving 2'-OH and triple-base interactions, as well as sodium ion coordination. Overall, the number of hydrogen bonds stabilizing the tertiary interactions exceeds the number involved in Watson-Crick base pairs. This structure will aid mechanistic analyses of ribosomal frameshifting.
 ==About this Structure==
@@ Line 12: / Line 12: @@
 Minor groove RNA triplex in the crystal structure of a ribosomal frameshifting viral pseudoknot., Su L, Chen L, Egli M, Berger JM, Rich A, Nat Struct Biol. 1999 Mar;6(3):285-92. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10074948 10074948]
 [[Category: Protein complex]]
-[[Category: Berger, J.M.]]
+[[Category: Berger, J M.]]
 [[Category: Chen, L.]]
 [[Category: Egli, M.]]
@@ Line 24: / Line 24: @@
 [[Category: viral frameshifting]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 21:43:27 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:12:11 2008''