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[[Image:2w7z.png|left|200px]]
==STRUCTURE OF THE PENTAPEPTIDE REPEAT PROTEIN EFSQNR, A DNA GYRASE INHIBITOR. FREE AMINES MODIFIED BY CYCLIC PENTYLATION WITH GLUTARALDEHYDE.==
<StructureSection load='2w7z' size='340' side='right' caption='[[2w7z]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2w7z]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W7Z OCA]. <br>
</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene><br>
<tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=LP6:6-PIPERIDIN-1-YL-L-NORLEUCINE'>LP6</scene></td></tr>
<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2w7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w7z OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2w7z RCSB], [http://www.ebi.ac.uk/pdbsum/2w7z PDBsum]</span></td></tr>
<table>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w7/2w7z_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The pentapeptide-repeat protein EfsQnr from Enterococcus faecalis protects DNA gyrase from inhibition by fluoroquinolones. EfsQnr was cloned and purified to homogeneity, but failed to produce diffraction-quality crystals in initial crystallization screens. Treatment of EfsQnr with glutaraldehyde and the strong reducing agent borane-dimethylamine resulted in a derivatized protein which produced crystals that diffracted to 1.6 A resolution; their structure was subsequently determined by single-wavelength anomalous dispersion. Analysis of the derivatized protein using Fourier transform ion cyclotron resonance mass spectrometry indicated a mass increase of 68 Da per free amino group. Electron-density maps about a limited number of structurally ordered lysines indicated that the modification was a cyclic pentylation of free amines, producing piperidine groups.


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Crystallization of a pentapeptide-repeat protein by reductive cyclic pentylation of free amines with glutaraldehyde.,Vetting MW, Hegde SS, Blanchard JS Acta Crystallogr D Biol Crystallogr. 2009 May;65(Pt 5):462-9. Epub 2009, Apr 18. PMID:19390151<ref>PMID:19390151</ref>
The line below this paragraph, containing "STRUCTURE_2w7z", creates the "Structure Box" on the page.
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{{STRUCTURE_2w7z|  PDB=2w7z  |  SCENE=  }}


===STRUCTURE OF THE PENTAPEPTIDE REPEAT PROTEIN EFSQNR, A DNA GYRASE INHIBITOR. FREE AMINES MODIFIED BY CYCLIC PENTYLATION WITH GLUTARALDEHYDE.===
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
 
</div>
 
== References ==
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<references/>
The line below this paragraph, {{ABSTRACT_PUBMED_19390151}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 19390151 is the PubMed ID number.
</StructureSection>
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{{ABSTRACT_PUBMED_19390151}}
 
==About this Structure==
[[2w7z]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W7Z OCA].
 
==Reference==
<ref group="xtra">PMID:019390151</ref><references group="xtra"/>
[[Category: Enterococcus faecalis]]
[[Category: Enterococcus faecalis]]
[[Category: Blanchard, J S.]]
[[Category: Blanchard, J S.]]
Retrieved from "https://proteopedia.org/2w7z"