464d: Difference between revisions

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==Overview==

An RNA helix with seven base pairs which was derived from the acceptor, stem of Escherichia coli tRNA(Ala), rGGGGCUA.rUAGCUCC (ALA(wt)), as well, as a variant, rGGGGCUA.rUAGCCCC (ALA(C70)), in which the single G.U wobble, base pair of ALA(wt) was replaced by G.C, crystallize in space group C2., Both non-isomorphic crystal forms display a complex packing pattern, which, can be described alternatively as disorder or pseudo-merohedral twinning., The structure of ALA(wt) was determined by SIRAS phasing using an, isomorphous iodine derivative, rGGGGCi(5)UA.rUAGCUCC (ALA(I)). All three, RNA structures were subsequently subjected to twin refinement in space, group P1, using anisotropic thermal displacement parameters at resolutions, of 1.16, 1.23 and 1.4 A for ALA(wt), ALA(I) and ALA(C70), respectively., Alternatively, the structure of ALA(wt) was refined in space group C2, assuming twofold disorder of the molecular orientation. The refined, structures are of reasonable quality according to all available, indicators. There are no systematic differences between the molecular, models resulting from twin refinement and disorder refinement.

An RNA helix with seven base pairs which was derived from the acceptor stem of Escherichia coli tRNA(Ala), rGGGGCUA.rUAGCUCC (ALA(wt)), as well as a variant, rGGGGCUA.rUAGCCCC (ALA(C70)), in which the single G.U wobble base pair of ALA(wt) was replaced by G.C, crystallize in space group C2. Both non-isomorphic crystal forms display a complex packing pattern, which can be described alternatively as disorder or pseudo-merohedral twinning. The structure of ALA(wt) was determined by SIRAS phasing using an isomorphous iodine derivative, rGGGGCi(5)UA.rUAGCUCC (ALA(I)). All three RNA structures were subsequently subjected to twin refinement in space group P1, using anisotropic thermal displacement parameters at resolutions of 1.16, 1.23 and 1.4 A for ALA(wt), ALA(I) and ALA(C70), respectively. Alternatively, the structure of ALA(wt) was refined in space group C2 assuming twofold disorder of the molecular orientation. The refined structures are of reasonable quality according to all available indicators. There are no systematic differences between the molecular models resulting from twin refinement and disorder refinement.

==About this Structure==

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[[Category: Herbst-Irmer, R.]]

[[Category: Mueller, U.]]

[[Category: Muller, Y.A.]]

[[Category: Muller, Y A.]]

[[Category: Sprinzl, M.]]

[[Category: NA]]

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[[Category: ribonucleic acid]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:12:28 2008''

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@@ Line 4: / Line 4: @@
 ==Overview==
-An RNA helix with seven base pairs which was derived from the acceptor, stem of Escherichia coli tRNA(Ala), rGGGGCUA.rUAGCUCC (ALA(wt)), as well, as a variant, rGGGGCUA.rUAGCCCC (ALA(C70)), in which the single G.U wobble, base pair of ALA(wt) was replaced by G.C, crystallize in space group C2., Both non-isomorphic crystal forms display a complex packing pattern, which, can be described alternatively as disorder or pseudo-merohedral twinning., The structure of ALA(wt) was determined by SIRAS phasing using an, isomorphous iodine derivative, rGGGGCi(5)UA.rUAGCUCC (ALA(I)). All three, RNA structures were subsequently subjected to twin refinement in space, group P1, using anisotropic thermal displacement parameters at resolutions, of 1.16, 1.23 and 1.4 A for ALA(wt), ALA(I) and ALA(C70), respectively., Alternatively, the structure of ALA(wt) was refined in space group C2, assuming twofold disorder of the molecular orientation. The refined, structures are of reasonable quality according to all available, indicators. There are no systematic differences between the molecular, models resulting from twin refinement and disorder refinement.
+An RNA helix with seven base pairs which was derived from the acceptor stem of Escherichia coli tRNA(Ala), rGGGGCUA.rUAGCUCC (ALA(wt)), as well as a variant, rGGGGCUA.rUAGCCCC (ALA(C70)), in which the single G.U wobble base pair of ALA(wt) was replaced by G.C, crystallize in space group C2. Both non-isomorphic crystal forms display a complex packing pattern, which can be described alternatively as disorder or pseudo-merohedral twinning. The structure of ALA(wt) was determined by SIRAS phasing using an isomorphous iodine derivative, rGGGGCi(5)UA.rUAGCUCC (ALA(I)). All three RNA structures were subsequently subjected to twin refinement in space group P1, using anisotropic thermal displacement parameters at resolutions of 1.16, 1.23 and 1.4 A for ALA(wt), ALA(I) and ALA(C70), respectively. Alternatively, the structure of ALA(wt) was refined in space group C2 assuming twofold disorder of the molecular orientation. The refined structures are of reasonable quality according to all available indicators. There are no systematic differences between the molecular models resulting from twin refinement and disorder refinement.
 ==About this Structure==
@@ Line 15: / Line 15: @@
 [[Category: Herbst-Irmer, R.]]
 [[Category: Mueller, U.]]
-[[Category: Muller, Y.A.]]
+[[Category: Muller, Y A.]]
 [[Category: Sprinzl, M.]]
 [[Category: NA]]
@@ Line 24: / Line 24: @@
 [[Category: ribonucleic acid]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 21:44:33 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:12:28 2008''