469d: Difference between revisions

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==Overview==

2'-O-(2-Methoxyethyl)-RNA (MOE-RNA) is a nucleic acid analog with, promising features for antisense applications. Compared with, phosphorothioate DNA (PS-DNA), the MOE modification offers improved, nuclease resistance, enhanced RNA affinity, improved cellular uptake and, intestinal absorption, reduced toxicity and immune stimulation. The, crystal structure of a fully modified MOE-RNA dodecamer duplex, (CGCGAAUUCGCG) was determined at 1.7 A resolution. In the majority of the, MOE substituents, the torsion angle around the ethylene alkyl chain, assumes a gauche conformation. The conformational preorganization of the, MOE groups is consistent with the improved RNA affinity and the extensive, hydration of the substituents could play a role in the improved cellular, uptake of MOE-RNA. A specific hydration pattern that bridges substituent, and phosphate oxygen atoms in the minor groove of MOE-RNA may explain its, high nuclease resistance.

2'-O-(2-Methoxyethyl)-RNA (MOE-RNA) is a nucleic acid analog with promising features for antisense applications. Compared with phosphorothioate DNA (PS-DNA), the MOE modification offers improved nuclease resistance, enhanced RNA affinity, improved cellular uptake and intestinal absorption, reduced toxicity and immune stimulation. The crystal structure of a fully modified MOE-RNA dodecamer duplex (CGCGAAUUCGCG) was determined at 1.7 A resolution. In the majority of the MOE substituents, the torsion angle around the ethylene alkyl chain assumes a gauche conformation. The conformational preorganization of the MOE groups is consistent with the improved RNA affinity and the extensive hydration of the substituents could play a role in the improved cellular uptake of MOE-RNA. A specific hydration pattern that bridges substituent and phosphate oxygen atoms in the minor groove of MOE-RNA may explain its high nuclease resistance.

==About this Structure==

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Crystal structure and improved antisense properties of 2'-O-(2-methoxyethyl)-RNA., Teplova M, Minasov G, Tereshko V, Inamati GB, Cook PD, Manoharan M, Egli M, Nat Struct Biol. 1999 Jun;6(6):535-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10360355 10360355]

[[Category: Protein complex]]

[[Category: Cook, P.D.]]

[[Category: Cook, P D.]]

[[Category: Egli, M.]]

[[Category: Inamati, G.]]

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[[Category: rna dodecamer duplex]]

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@@ Line 4: / Line 4: @@
 ==Overview==
-'-O-(2-Methoxyethyl)-RNA (MOE-RNA) is a nucleic acid analog with, promising features for antisense applications. Compared with, phosphorothioate DNA (PS-DNA), the MOE modification offers improved, nuclease resistance, enhanced RNA affinity, improved cellular uptake and, intestinal absorption, reduced toxicity and immune stimulation. The, crystal structure of a fully modified MOE-RNA dodecamer duplex, (CGCGAAUUCGCG) was determined at 1.7 A resolution. In the majority of the, MOE substituents, the torsion angle around the ethylene alkyl chain, assumes a gauche conformation. The conformational preorganization of the, MOE groups is consistent with the improved RNA affinity and the extensive, hydration of the substituents could play a role in the improved cellular, uptake of MOE-RNA. A specific hydration pattern that bridges substituent, and phosphate oxygen atoms in the minor groove of MOE-RNA may explain its, high nuclease resistance.
+'-O-(2-Methoxyethyl)-RNA (MOE-RNA) is a nucleic acid analog with promising features for antisense applications. Compared with phosphorothioate DNA (PS-DNA), the MOE modification offers improved nuclease resistance, enhanced RNA affinity, improved cellular uptake and intestinal absorption, reduced toxicity and immune stimulation. The crystal structure of a fully modified MOE-RNA dodecamer duplex (CGCGAAUUCGCG) was determined at 1.7 A resolution. In the majority of the MOE substituents, the torsion angle around the ethylene alkyl chain assumes a gauche conformation. The conformational preorganization of the MOE groups is consistent with the improved RNA affinity and the extensive hydration of the substituents could play a role in the improved cellular uptake of MOE-RNA. A specific hydration pattern that bridges substituent and phosphate oxygen atoms in the minor groove of MOE-RNA may explain its high nuclease resistance.
 ==About this Structure==
@@ Line 12: / Line 12: @@
 Crystal structure and improved antisense properties of 2'-O-(2-methoxyethyl)-RNA., Teplova M, Minasov G, Tereshko V, Inamati GB, Cook PD, Manoharan M, Egli M, Nat Struct Biol. 1999 Jun;6(6):535-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10360355 10360355]
 [[Category: Protein complex]]
-[[Category: Cook, P.D.]]
+[[Category: Cook, P D.]]
 [[Category: Egli, M.]]
 [[Category: Inamati, G.]]
@@ Line 22: / Line 22: @@
 [[Category: rna dodecamer duplex]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 21:44:45 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:12:32 2008''