2ivf: Difference between revisions

Line 1:

[[Image:~~2ivf~~.~~png~~|~~left~~|~~200px~~]]

==ETHYLBENZENE DEHYDROGENASE FROM AROMATOLEUM AROMATICUM==

== Structural highlights ==

[[2ivf]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Aromatoleum_aromaticum Aromatoleum aromaticum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IVF OCA]. <br>

<b>Activity:</b> <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span><br>

== Evolutionary Conservation ==

[[Image:Consurf_key_small.gif|right]]

Check<jmol>

<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/iv/2ivf_consurf.spt"</scriptWhenChecked>

<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>

<text>to colour the structure by Evolutionary Conservation</text>

</jmolCheckbox>

</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].

== Publication Abstract from PubMed ==

Anaerobic degradation of hydrocarbons was discovered a decade ago, and ethylbenzene dehydrogenase was one of the first characterized enzymes involved. The structure of the soluble periplasmic 165 kDa enzyme was established at 1.88 A resolution. It is a heterotrimer. The alpha subunit contains the catalytic center with a molybdenum held by two molybdopterin-guanine dinucleotides, one with an open pyran ring, and an iron-sulfur cluster with a histidine ligand. During catalysis, electrons produced by substrate oxidation are transferred to a heme in the gamma subunit and then presumably to a separate cytochrome involved in nitrate respiration. The beta subunit contains four iron-sulfur clusters and is structurally related to ferredoxins. The gamma subunit is the first known protein with a methionine and a lysine as axial heme ligands. The catalytic product was modeled into the active center, showing the reaction geometry. A mechanism consistent with activity and inhibition data of ethylbenzene-related compounds is proposed.

~~<!--~~

Crystal structure of ethylbenzene dehydrogenase from Aromatoleum aromaticum.,Kloer DP, Hagel C, Heider J, Schulz GE Structure. 2006 Sep;14(9):1377-88. PMID:16962969<ref>PMID:16962969</ref>

~~The line below this paragraph~~, ~~containing "STRUCTURE_2ivf"~~, ~~creates the "~~Structure ~~Box" on the page~~.

~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)

~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~

~~or leave the SCENE parameter empty for the default display~~.

-->

~~{{STRUCTURE_2ivf| PDB=2ivf | SCENE= }}~~

~~===ETHYLBENZENE DEHYDROGENASE FROM AROMATOLEUM AROMATICUM===~~

From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>

== References ==

~~<!--~~

__TOC__

~~The line below this paragraph~~, ~~{{ABSTRACT_PUBMED_16962969}}, adds the Publication Abstract to~~ the ~~page~~

</StructureSection>

~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 16962969 is the PubMed ID number~~.

-->

~~{{ABSTRACT_PUBMED_16962969}}~~

==~~About this Structure~~==

~~[[2ivf]] is a 3 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Aromatoleum_aromaticum Aromatoleum aromaticum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IVF OCA].~~

~~==Reference==~~

~~<ref group="xtra">PMID:016962969~~</~~ref><references group="xtra"/~~>

[[Category: Aromatoleum aromaticum]]

[[Category: Ethylbenzene hydroxylase]]

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@@ Line 1: / Line 1: @@
-[[Image:2ivf.png|left|200px]]
+==ETHYLBENZENE DEHYDROGENASE FROM AROMATOLEUM AROMATICUM==
+<StructureSection load='2ivf' size='340' side='right' caption='[[2ivf]], [[Resolution|resolution]] 1.88&Aring;' scene=''>
+== Structural highlights ==
+[[2ivf]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Aromatoleum_aromaticum Aromatoleum aromaticum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IVF OCA]. <br>
+<b>Activity:</b> <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span><br>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/iv/2ivf_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
+<div style="clear:both"></div>
+== Publication Abstract from PubMed ==
+Anaerobic degradation of hydrocarbons was discovered a decade ago, and ethylbenzene dehydrogenase was one of the first characterized enzymes involved. The structure of the soluble periplasmic 165 kDa enzyme was established at 1.88 A resolution. It is a heterotrimer. The alpha subunit contains the catalytic center with a molybdenum held by two molybdopterin-guanine dinucleotides, one with an open pyran ring, and an iron-sulfur cluster with a histidine ligand. During catalysis, electrons produced by substrate oxidation are transferred to a heme in the gamma subunit and then presumably to a separate cytochrome involved in nitrate respiration. The beta subunit contains four iron-sulfur clusters and is structurally related to ferredoxins. The gamma subunit is the first known protein with a methionine and a lysine as axial heme ligands. The catalytic product was modeled into the active center, showing the reaction geometry. A mechanism consistent with activity and inhibition data of ethylbenzene-related compounds is proposed.
-<!--
+Crystal structure of ethylbenzene dehydrogenase from Aromatoleum aromaticum.,Kloer DP, Hagel C, Heider J, Schulz GE Structure. 2006 Sep;14(9):1377-88. PMID:16962969<ref>PMID:16962969</ref>
-The line below this paragraph, containing "STRUCTURE_2ivf", creates the "Structure Box" on the page.
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-or leave the SCENE parameter empty for the default display.
--->
-{{STRUCTURE_2ivf|  PDB=2ivf  |  SCENE=  }}
-===ETHYLBENZENE DEHYDROGENASE FROM AROMATOLEUM AROMATICUM===
+From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
+== References ==
+<references/>
-<!--
+__TOC__
-The line below this paragraph, {{ABSTRACT_PUBMED_16962969}}, adds the Publication Abstract to the page
+</StructureSection>
-(as it appears on PubMed at http://www.pubmed.gov), where 16962969 is the PubMed ID number.
--->
-{{ABSTRACT_PUBMED_16962969}}
-==About this Structure==
-[[2ivf]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Aromatoleum_aromaticum Aromatoleum aromaticum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IVF OCA].
-==Reference==
-<ref group="xtra">PMID:016962969</ref><references group="xtra"/>
 [[Category: Aromatoleum aromaticum]]
 [[Category: Ethylbenzene hydroxylase]]