2qqr: Difference between revisions

The lysine demethylase JMJD2A has the unique property of binding, trimethylated peptides from two different histone sequences (H3K4me3 and, H4K20me3) through its tudor domains. Here we show using X-ray, crystallography and calorimetry that H3K4me3 and H4K20me3, which are, recognized with similar affinities by JMJD2A, adopt radically different, binding modes, to the extent that we were able to design single point, mutations in JMJD2A that inhibited the recognition of H3K4me3 but not, H4K20me3 and vice versa.

2QQR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Sites: <scene name='pdbsite=AC1:So4 Binding Site For Residue A 1'>AC1</scene>, <scene name='pdbsite=AC2:So4 Binding Site For Residue B 2'>AC2</scene>, <scene name='pdbsite=AC3:So4 Binding Site For Residue A 3'>AC3</scene>, <scene name='pdbsite=AC4:So4 Binding Site For Residue B 4'>AC4</scene>, <scene name='pdbsite=AC5:So4 Binding Site For Residue B 5'>AC5</scene> and <scene name='pdbsite=AC6:So4 Binding Site For Residue A 6'>AC6</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QQR OCA].  

[[Category: Botuyan, M.V.]]

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