3bd9: Difference between revisions
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New page: left|200px<br /><applet load="3bd9" size="350" color="white" frame="true" align="right" spinBox="true" caption="3bd9, resolution 2.30Å" /> '''human 3-O-sulfotrans... |
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caption="3bd9, resolution 2.30Å" /> | caption="3bd9, resolution 2.30Å" /> | ||
'''human 3-O-sulfotransferase isoform 5 with bound PAP'''<br /> | '''human 3-O-sulfotransferase isoform 5 with bound PAP'''<br /> | ||
==Overview== | |||
The biosynthesis of heparan sulfate (HS) involves an array of specialized sulfotransferases. Here, we present a study aimed at engineering the substrate specificity of different HS 3-O-sulfotransferase isoforms. Based on the crystal structures, we identified a pair of amino acid residues responsible for selecting the substrates. Mutations of these residues altered the substrate specificities. Our results demonstrate the feasibility of tailoring the specificity of sulfotransferases to modify HS with desired functions. | |||
==About this Structure== | ==About this Structure== | ||
3BD9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=A3P:'>A3P</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/[Heparan_sulfate]-glucosamine_3-sulfotransferase_1 [Heparan sulfate]-glucosamine 3-sulfotransferase 1], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.2.23 2.8.2.23] Known structural/functional Site: <scene name='pdbsite=AC1:A3p Binding Site For Residue A 347'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BD9 OCA]. | 3BD9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=A3P:'>A3P</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/[Heparan_sulfate]-glucosamine_3-sulfotransferase_1 [Heparan sulfate]-glucosamine 3-sulfotransferase 1], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.2.23 2.8.2.23] Known structural/functional Site: <scene name='pdbsite=AC1:A3p+Binding+Site+For+Residue+A+347'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BD9 OCA]. | ||
==Reference== | |||
Engineering sulfotransferases to modify heparan sulfate., Xu D, Moon AF, Song D, Pedersen LC, Liu J, Nat Chem Biol. 2008 Mar;4(3):200-2. Epub 2008 Jan 27. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18223645 18223645] | |||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: [Heparan sulfate]-glucosamine 3-sulfotransferase 1]] | [[Category: [Heparan sulfate]-glucosamine 3-sulfotransferase 1]] | ||
[[Category: Liu, J.]] | [[Category: Liu, J.]] | ||
[[Category: Moon, A | [[Category: Moon, A F.]] | ||
[[Category: Pedersen, L | [[Category: Pedersen, L C.]] | ||
[[Category: Song, D.]] | [[Category: Song, D.]] | ||
[[Category: Xu, D.]] | [[Category: Xu, D.]] | ||
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[[Category: transmembrane]] | [[Category: transmembrane]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:04:58 2008'' | ||
