TolA: Difference between revisions
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{{STRUCTURE_1s62| PDB=1s62 | SIZE=400| SCENE=TolA/Ctdtola/1|right|CAPTION=E. coli | {{STRUCTURE_1s62| PDB=1s62 | SIZE=400| SCENE=TolA/Ctdtola/1|right|CAPTION=E. coli TolA C-terminal [[1s62]] }} | ||
==Structure== | ==Structure== | ||
TolA is located in the inner membrane and comprises of three domains: the N-terminal domain I (TolAI), from residues 1-47 including a 20-residue hydrophobic membrane spanning region which anchors the protein to the cytoplasmic membrane<ref name='Lazzaroni'>PMID: 7853390</ref>; domain II (TolAII), from residues 48-301, which forms a rigid helix connecting the domains either side of it; and the C-terminal domain III (TolAIII) from residues 302-421, which may be involved in the function of TolA by interacting with the periplasmic or outer membrane proteins, due to the tethering to domain II<ref name='Sharyn'>PMID: 8416897</ref>. | TolA is located in the inner membrane and comprises of three domains: the N-terminal domain I (TolAI), from residues 1-47 including a 20-residue hydrophobic membrane spanning region which anchors the protein to the cytoplasmic membrane<ref name='Lazzaroni'>PMID: 7853390</ref>; domain II (TolAII), from residues 48-301, which forms a rigid helix connecting the domains either side of it; and the C-terminal domain III (TolAIII) from residues 302-421, which may be involved in the function of TolA by interacting with the periplasmic or outer membrane proteins, due to the tethering to domain II<ref name='Sharyn'>PMID: 8416897</ref>. | ||
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* The other colicins require additional receptors in order to bind initially to the outer membrane | * The other colicins require additional receptors in order to bind initially to the outer membrane | ||
*Colicin E1 also does not require as high of TolA levels than the other colicins in order for translocation to occur | *Colicin E1 also does not require as high of TolA levels than the other colicins in order for translocation to occur | ||
==3D structures of TolA== | |||
[[1lr0]] – TolA domain III – ''Pseudomonas aeruginosa''<br /> | |||
[[1s62]] – EcTolA C terminal – ''Escherichia coli'' – NMR<br /> | |||
[[2x9a]] - EcTolA C terminal + attachment protein G3P | |||
==References== | ==References== | ||
<references/> | <references/> | ||
