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==Overview==

The X-ray crystal structure of, 7,8-dihydro-6-hydroxymethylpterinpyrophosphokinase (PPPK) in a ternary, complex with ATP and a pterin analogue has been solved to 2.0 A, resolution, giving, for the first time, detailed information of the, PPPK/ATP intermolecular interactions and the accompanying conformational, change. The first 100 residues of the 158 residue peptide contain a, betaalpha betabeta alphabeta motif present in several other proteins, including nucleoside diphosphate kinase. Comparative sequence examination, of a wide range of prokaryotic and lower eukaryotic species confirms the, conservation of the PPPK active site, indicating the value of this de novo, folate biosynthesis pathway enzyme as a potential target for the, development of novel broad-spectrum anti-infective agents.

The X-ray crystal structure of 7,8-dihydro-6-hydroxymethylpterinpyrophosphokinase (PPPK) in a ternary complex with ATP and a pterin analogue has been solved to 2.0 A resolution, giving, for the first time, detailed information of the PPPK/ATP intermolecular interactions and the accompanying conformational change. The first 100 residues of the 158 residue peptide contain a betaalpha betabeta alphabeta motif present in several other proteins including nucleoside diphosphate kinase. Comparative sequence examination of a wide range of prokaryotic and lower eukaryotic species confirms the conservation of the PPPK active site, indicating the value of this de novo folate biosynthesis pathway enzyme as a potential target for the development of novel broad-spectrum anti-infective agents.

==About this Structure==

Line 15:

[[Category: Single protein]]

[[Category: Achari, A.]]

[[Category: Bryant, P.K.]]

[[Category: Bryant, P K.]]

[[Category: Champness, J.N.]]

[[Category: Champness, J N.]]

[[Category: Rosemond, J.]]

[[Category: Scott, D.L.]]

[[Category: Scott, D L.]]

[[Category: Somers, D.O.]]

[[Category: Somers, D O.]]

[[Category: Stammers, D.K.]]

[[Category: Stammers, D K.]]

[[Category: 87Y]]

[[Category: ATP]]

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[[Category: pyrophosphorylase]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:21:42 2008''

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@@ Line 4: / Line 4: @@
 ==Overview==
-The X-ray crystal structure of, 7,8-dihydro-6-hydroxymethylpterinpyrophosphokinase (PPPK) in a ternary, complex with ATP and a pterin analogue has been solved to 2.0 A, resolution, giving, for the first time, detailed information of the, PPPK/ATP intermolecular interactions and the accompanying conformational, change. The first 100 residues of the 158 residue peptide contain a, betaalpha betabeta alphabeta motif present in several other proteins, including nucleoside diphosphate kinase. Comparative sequence examination, of a wide range of prokaryotic and lower eukaryotic species confirms the, conservation of the PPPK active site, indicating the value of this de novo, folate biosynthesis pathway enzyme as a potential target for the, development of novel broad-spectrum anti-infective agents.
+The X-ray crystal structure of 7,8-dihydro-6-hydroxymethylpterinpyrophosphokinase (PPPK) in a ternary complex with ATP and a pterin analogue has been solved to 2.0 A resolution, giving, for the first time, detailed information of the PPPK/ATP intermolecular interactions and the accompanying conformational change. The first 100 residues of the 158 residue peptide contain a betaalpha betabeta alphabeta motif present in several other proteins including nucleoside diphosphate kinase. Comparative sequence examination of a wide range of prokaryotic and lower eukaryotic species confirms the conservation of the PPPK active site, indicating the value of this de novo folate biosynthesis pathway enzyme as a potential target for the development of novel broad-spectrum anti-infective agents.
 ==About this Structure==
@@ Line 15: / Line 15: @@
 [[Category: Single protein]]
 [[Category: Achari, A.]]
-[[Category: Bryant, P.K.]]
+[[Category: Bryant, P K.]]
-[[Category: Champness, J.N.]]
+[[Category: Champness, J N.]]
 [[Category: Rosemond, J.]]
-[[Category: Scott, D.L.]]
+[[Category: Scott, D L.]]
-[[Category: Somers, D.O.]]
+[[Category: Somers, D O.]]
-[[Category: Stammers, D.K.]]
+[[Category: Stammers, D K.]]
 [[Category: 87Y]]
 [[Category: ATP]]
@@ Line 27: / Line 27: @@
 [[Category: pyrophosphorylase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb  3 09:35:59 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:21:42 2008''