1dz9: Difference between revisions

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==Overview==

Members of the cytochrome P450 superfamily catalyze the addition of, molecular oxygen to nonactivated hydrocarbons at physiological, temperature-a reaction that requires high temperature to proceed in the, absence of a catalyst. Structures were obtained for three intermediates in, the hydroxylation reaction of camphor by P450cam with trapping techniques, and cryocrystallography. The structure of the ferrous dioxygen adduct of, P450cam was determined with 0.91 angstrom wavelength x-rays; irradiation, with 1.5 angstrom x-rays results in breakdown of the dioxygen molecule to, an intermediate that would be consistent with an oxyferryl species. The, structures show conformational changes in several important residues and, reveal a network of bound water molecules that may provide the protons, needed for the reaction.

Members of the cytochrome P450 superfamily catalyze the addition of molecular oxygen to nonactivated hydrocarbons at physiological temperature-a reaction that requires high temperature to proceed in the absence of a catalyst. Structures were obtained for three intermediates in the hydroxylation reaction of camphor by P450cam with trapping techniques and cryocrystallography. The structure of the ferrous dioxygen adduct of P450cam was determined with 0.91 angstrom wavelength x-rays; irradiation with 1.5 angstrom x-rays results in breakdown of the dioxygen molecule to an intermediate that would be consistent with an oxyferryl species. The structures show conformational changes in several important residues and reveal a network of bound water molecules that may provide the protons needed for the reaction.

==About this Structure==

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[[Category: Pseudomonas putida]]

[[Category: Single protein]]

[[Category: Benson, D.E.]]

[[Category: Benson, D E.]]

[[Category: Berendzen, J.]]

[[Category: Chu, K.]]

[[Category: Maves, S.A.]]

[[Category: Maves, S A.]]

[[Category: Petsko, G.A.]]

[[Category: Petsko, G A.]]

[[Category: Ringe, D.]]

[[Category: Schlichting, I.]]

[[Category: Sligar, S.G.]]

[[Category: Sligar, S G.]]

[[Category: Stock, A.M.]]

[[Category: Stock, A M.]]

[[Category: Sweet, R.M.]]

[[Category: Sweet, R M.]]

[[Category: CAM]]

[[Category: HEM]]

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[[Category: reaction intermediate]]

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@@ Line 4: / Line 4: @@
 ==Overview==
-Members of the cytochrome P450 superfamily catalyze the addition of, molecular oxygen to nonactivated hydrocarbons at physiological, temperature-a reaction that requires high temperature to proceed in the, absence of a catalyst. Structures were obtained for three intermediates in, the hydroxylation reaction of camphor by P450cam with trapping techniques, and cryocrystallography. The structure of the ferrous dioxygen adduct of, P450cam was determined with 0.91 angstrom wavelength x-rays; irradiation, with 1.5 angstrom x-rays results in breakdown of the dioxygen molecule to, an intermediate that would be consistent with an oxyferryl species. The, structures show conformational changes in several important residues and, reveal a network of bound water molecules that may provide the protons, needed for the reaction.
+Members of the cytochrome P450 superfamily catalyze the addition of molecular oxygen to nonactivated hydrocarbons at physiological temperature-a reaction that requires high temperature to proceed in the absence of a catalyst. Structures were obtained for three intermediates in the hydroxylation reaction of camphor by P450cam with trapping techniques and cryocrystallography. The structure of the ferrous dioxygen adduct of P450cam was determined with 0.91 angstrom wavelength x-rays; irradiation with 1.5 angstrom x-rays results in breakdown of the dioxygen molecule to an intermediate that would be consistent with an oxyferryl species. The structures show conformational changes in several important residues and reveal a network of bound water molecules that may provide the protons needed for the reaction.
 ==About this Structure==
@@ Line 13: / Line 13: @@
 [[Category: Pseudomonas putida]]
 [[Category: Single protein]]
-[[Category: Benson, D.E.]]
+[[Category: Benson, D E.]]
 [[Category: Berendzen, J.]]
 [[Category: Chu, K.]]
-[[Category: Maves, S.A.]]
+[[Category: Maves, S A.]]
-[[Category: Petsko, G.A.]]
+[[Category: Petsko, G A.]]
 [[Category: Ringe, D.]]
 [[Category: Schlichting, I.]]
-[[Category: Sligar, S.G.]]
+[[Category: Sligar, S G.]]
-[[Category: Stock, A.M.]]
+[[Category: Stock, A M.]]
-[[Category: Sweet, R.M.]]
+[[Category: Sweet, R M.]]
 [[Category: CAM]]
 [[Category: HEM]]
@@ Line 32: / Line 32: @@
 [[Category: reaction intermediate]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb  3 09:36:12 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:22:05 2008''