1gvg: Difference between revisions
No edit summary |
No edit summary |
||
| Line 4: | Line 4: | ||
==Overview== | ==Overview== | ||
Clavaminate synthase (CAS), a 2-oxoglutarate (2OG) dependent dioxygenase, catalyses three steps in the biosynthesis of clavulanic acid. Crystals of | Clavaminate synthase (CAS), a 2-oxoglutarate (2OG) dependent dioxygenase, catalyses three steps in the biosynthesis of clavulanic acid. Crystals of CAS complexed with Fe(II), 2OG and deoxyguanidinoproclavaminate were exposed to nitric oxide (NO) acting as a dioxygen analogue. Prior to exposure with NO, the active site Fe(II) is octahedrally coordinated by a water molecule, the 2-oxo and 1-carboxylate groups of 2OG, and the side-chains of an aspartyl and two histidinyl residues. NO binds to the position previously occupied by the 2OG 1-carboxylate concomitant with rearrangement of the latter to the position previously occupied by the displaced water. | ||
==About this Structure== | ==About this Structure== | ||
| Line 13: | Line 13: | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Streptomyces clavuligerus]] | [[Category: Streptomyces clavuligerus]] | ||
[[Category: Clifton, I | [[Category: Clifton, I J.]] | ||
[[Category: Harlos, K.]] | [[Category: Harlos, K.]] | ||
[[Category: Mckinnon, C | [[Category: Mckinnon, C H.]] | ||
[[Category: Ren, J.]] | [[Category: Ren, J.]] | ||
[[Category: Schofield, C | [[Category: Schofield, C J.]] | ||
[[Category: Zhang, Z | [[Category: Zhang, Z H.]] | ||
[[Category: AKG]] | [[Category: AKG]] | ||
[[Category: FE]] | [[Category: FE]] | ||
| Line 32: | Line 32: | ||
[[Category: trifunctional enzyme]] | [[Category: trifunctional enzyme]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:54:23 2008'' | ||
Revision as of 10:54, 21 February 2008
|
CRYSTAL STRUCTURE OF CLAVAMINATE SYNTHASE WITH NITRIC OXIDE
Overview
Clavaminate synthase (CAS), a 2-oxoglutarate (2OG) dependent dioxygenase, catalyses three steps in the biosynthesis of clavulanic acid. Crystals of CAS complexed with Fe(II), 2OG and deoxyguanidinoproclavaminate were exposed to nitric oxide (NO) acting as a dioxygen analogue. Prior to exposure with NO, the active site Fe(II) is octahedrally coordinated by a water molecule, the 2-oxo and 1-carboxylate groups of 2OG, and the side-chains of an aspartyl and two histidinyl residues. NO binds to the position previously occupied by the 2OG 1-carboxylate concomitant with rearrangement of the latter to the position previously occupied by the displaced water.
About this Structure
1GVG is a Single protein structure of sequence from Streptomyces clavuligerus with FE, SO4, NO, AKG and PCX as ligands. Known structural/functional Site: AC1. Full crystallographic information is available from OCA.
Reference
Crystal structure of a clavaminate synthase-Fe(II)-2-oxoglutarate-substrate-NO complex: evidence for metal centered rearrangements., Zhang Z, Ren J, Harlos K, McKinnon CH, Clifton IJ, Schofield CJ, FEBS Lett. 2002 Apr 24;517(1-3):7-12. PMID:12062399
Page seeded by OCA on Thu Feb 21 12:54:23 2008
