1h0d: Difference between revisions

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==Overview==

The murine monoclonal antibody 26-2F neutralizes the angiogenic and, ribonucleolytic activities of human angiogenin (ANG) and is highly, effective in preventing the establishment and metastatic dissemination of, human tumors in athymic mice. Here we report a 2.0 A resolution crystal, structure for the complex of ANG with the Fab fragment of 26-2F that, reveals the detailed interactions between ANG and the, complementarity-determining regions (CDRs) of the antibody. Surprisingly, Fab binding induces a dramatic conformational change in the cell binding, region of ANG at the opposite end of the molecule from the combining site;, crosslinking experiments indicate that this rearrangement also occurs in, solution. The ANG-Fab complex structure should be invaluable for designing, maximally humanized versions of 26-2F for potential clinical use.

The murine monoclonal antibody 26-2F neutralizes the angiogenic and ribonucleolytic activities of human angiogenin (ANG) and is highly effective in preventing the establishment and metastatic dissemination of human tumors in athymic mice. Here we report a 2.0 A resolution crystal structure for the complex of ANG with the Fab fragment of 26-2F that reveals the detailed interactions between ANG and the complementarity-determining regions (CDRs) of the antibody. Surprisingly, Fab binding induces a dramatic conformational change in the cell binding region of ANG at the opposite end of the molecule from the combining site; crosslinking experiments indicate that this rearrangement also occurs in solution. The ANG-Fab complex structure should be invaluable for designing maximally humanized versions of 26-2F for potential clinical use.

==Disease==

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[[Category: Homo sapiens]]

[[Category: Single protein]]

[[Category: Acharya, K.R.]]

[[Category: Acharya, K R.]]

[[Category: Chavali, G.B.]]

[[Category: Chavali, G B.]]

[[Category: Papageorgiou, A.C.]]

[[Category: Papageorgiou, A C.]]

[[Category: GOL]]

[[Category: SO4]]

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[[Category: ribonuclease]]

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@@ Line 4: / Line 4: @@
 ==Overview==
-The murine monoclonal antibody 26-2F neutralizes the angiogenic and, ribonucleolytic activities of human angiogenin (ANG) and is highly, effective in preventing the establishment and metastatic dissemination of, human tumors in athymic mice. Here we report a 2.0 A resolution crystal, structure for the complex of ANG with the Fab fragment of 26-2F that, reveals the detailed interactions between ANG and the, complementarity-determining regions (CDRs) of the antibody. Surprisingly, Fab binding induces a dramatic conformational change in the cell binding, region of ANG at the opposite end of the molecule from the combining site;, crosslinking experiments indicate that this rearrangement also occurs in, solution. The ANG-Fab complex structure should be invaluable for designing, maximally humanized versions of 26-2F for potential clinical use.
+The murine monoclonal antibody 26-2F neutralizes the angiogenic and ribonucleolytic activities of human angiogenin (ANG) and is highly effective in preventing the establishment and metastatic dissemination of human tumors in athymic mice. Here we report a 2.0 A resolution crystal structure for the complex of ANG with the Fab fragment of 26-2F that reveals the detailed interactions between ANG and the complementarity-determining regions (CDRs) of the antibody. Surprisingly, Fab binding induces a dramatic conformational change in the cell binding region of ANG at the opposite end of the molecule from the combining site; crosslinking experiments indicate that this rearrangement also occurs in solution. The ANG-Fab complex structure should be invaluable for designing maximally humanized versions of 26-2F for potential clinical use.
 ==Disease==
@@ Line 16: / Line 16: @@
 [[Category: Homo sapiens]]
 [[Category: Single protein]]
-[[Category: Acharya, K.R.]]
+[[Category: Acharya, K R.]]
-[[Category: Chavali, G.B.]]
+[[Category: Chavali, G B.]]
-[[Category: Papageorgiou, A.C.]]
+[[Category: Papageorgiou, A C.]]
 [[Category: GOL]]
 [[Category: SO4]]
@@ Line 24: / Line 24: @@
 [[Category: ribonuclease]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb  3 09:44:45 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:56:01 2008''