1odg: Difference between revisions

Line 4:

==Overview==

Very-short-patch repair (Vsr) enzymes occur in a variety of bacteria, where they initiate nucleotide excision repair of G:T mismatches arising, by deamination of 5-methyl-cytosines in specific regulatory sequences. We, have now determined the structure of the archetypal dcm-Vsr endonuclease, from Escherichia coli bound to the cleaved authentic, hemi-deaminated/hemi-methylated dcm sequence 5'-C-OH-3', 5'-p-T-p-A-p-G-p-G-3'/3'-G-p-G-p-T-p(Me5)C-p-C formed by self-assembly of, a 12mer oligonucleotide into a continuous nicked DNA superhelix. The, structure reveals the presence of a Hoogsteen base pair within the, deaminated recognition sequence and the substantial distortions of the DNA, that accompany Vsr binding to product sites.

Very-short-patch repair (Vsr) enzymes occur in a variety of bacteria, where they initiate nucleotide excision repair of G:T mismatches arising by deamination of 5-methyl-cytosines in specific regulatory sequences. We have now determined the structure of the archetypal dcm-Vsr endonuclease from Escherichia coli bound to the cleaved authentic hemi-deaminated/hemi-methylated dcm sequence 5'-C-OH-3' 5'-p-T-p-A-p-G-p-G-3'/3'-G-p-G-p-T-p(Me5)C-p-C formed by self-assembly of a 12mer oligonucleotide into a continuous nicked DNA superhelix. The structure reveals the presence of a Hoogsteen base pair within the deaminated recognition sequence and the substantial distortions of the DNA that accompany Vsr binding to product sites.

==About this Structure==

Line 14:

[[Category: Protein complex]]

[[Category: Brown, T.]]

[[Category: Bunting, K.A.]]

[[Category: Bunting, K A.]]

[[Category: Headley, A.]]

[[Category: Pearl, L.H.]]

[[Category: Pearl, L H.]]

[[Category: Roe, S.M.]]

[[Category: Roe, S M.]]

[[Category: Savva, R.]]

[[Category: ZN]]

Line 28:

[[Category: zinc]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun~~ Feb ~~3 09~~:56:30 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:16:25 2008''

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 4: / Line 4: @@
 ==Overview==
-Very-short-patch repair (Vsr) enzymes occur in a variety of bacteria, where they initiate nucleotide excision repair of G:T mismatches arising, by deamination of 5-methyl-cytosines in specific regulatory sequences. We, have now determined the structure of the archetypal dcm-Vsr endonuclease, from Escherichia coli bound to the cleaved authentic, hemi-deaminated/hemi-methylated dcm sequence 5'-C-OH-3', 5'-p-T-p-A-p-G-p-G-3'/3'-G-p-G-p-T-p(Me5)C-p-C formed by self-assembly of, a 12mer oligonucleotide into a continuous nicked DNA superhelix. The, structure reveals the presence of a Hoogsteen base pair within the, deaminated recognition sequence and the substantial distortions of the DNA, that accompany Vsr binding to product sites.
+Very-short-patch repair (Vsr) enzymes occur in a variety of bacteria, where they initiate nucleotide excision repair of G:T mismatches arising by deamination of 5-methyl-cytosines in specific regulatory sequences. We have now determined the structure of the archetypal dcm-Vsr endonuclease from Escherichia coli bound to the cleaved authentic hemi-deaminated/hemi-methylated dcm sequence 5'-C-OH-3' 5'-p-T-p-A-p-G-p-G-3'/3'-G-p-G-p-T-p(Me5)C-p-C formed by self-assembly of a 12mer oligonucleotide into a continuous nicked DNA superhelix. The structure reveals the presence of a Hoogsteen base pair within the deaminated recognition sequence and the substantial distortions of the DNA that accompany Vsr binding to product sites.
 ==About this Structure==
@@ Line 14: / Line 14: @@
 [[Category: Protein complex]]
 [[Category: Brown, T.]]
-[[Category: Bunting, K.A.]]
+[[Category: Bunting, K A.]]
 [[Category: Headley, A.]]
-[[Category: Pearl, L.H.]]
+[[Category: Pearl, L H.]]
-[[Category: Roe, S.M.]]
+[[Category: Roe, S M.]]
 [[Category: Savva, R.]]
 [[Category: ZN]]
@@ Line 28: / Line 28: @@
 [[Category: zinc]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb  3 09:56:30 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:16:25 2008''