1ohl: Difference between revisions

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==Overview==

The X-ray structure of yeast 5-aminolaevulinic acid dehydratase, in which, the catalytic site of the enzyme is complexed with a putative cyclic, intermediate composed of both substrate moieties, has been solved at 0.16, nm (1.6 A) resolution. The cyclic intermediate is bound covalently to, Lys(263) with the amino group of the aminomethyl side chain ligated to the, active-site zinc ion in a position normally occupied by a catalytic, hydroxide ion. The cyclic intermediate is catalytically competent, as, shown by its turnover in the presence of added substrate to form, porphobilinogen. The findings, combined with those of previous studies, are consistent with a catalytic mechanism in which the C-C bond linking, both substrates in the intermediate is formed before the C-N bond.

The X-ray structure of yeast 5-aminolaevulinic acid dehydratase, in which the catalytic site of the enzyme is complexed with a putative cyclic intermediate composed of both substrate moieties, has been solved at 0.16 nm (1.6 A) resolution. The cyclic intermediate is bound covalently to Lys(263) with the amino group of the aminomethyl side chain ligated to the active-site zinc ion in a position normally occupied by a catalytic hydroxide ion. The cyclic intermediate is catalytically competent, as shown by its turnover in the presence of added substrate to form porphobilinogen. The findings, combined with those of previous studies, are consistent with a catalytic mechanism in which the C-C bond linking both substrates in the intermediate is formed before the C-N bond.

==About this Structure==

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[[Category: Saccharomyces cerevisiae]]

[[Category: Single protein]]

[[Category: Brindley, A.A.]]

[[Category: Brindley, A A.]]

[[Category: Butler, D.]]

[[Category: Coates, L.]]

[[Category: Cooper, J.B.]]

[[Category: Cooper, J B.]]

[[Category: Erskine, P.T.]]

[[Category: Erskine, P T.]]

[[Category: Shoolingin-Jordan, P.M.]]

[[Category: Shoolingin-Jordan, P M.]]

[[Category: Warren, M.J.]]

[[Category: Warren, M J.]]

[[Category: Wood, S.P.]]

[[Category: Wood, S P.]]

[[Category: Youell, J.H.]]

[[Category: Youell, J H.]]

[[Category: BME]]

[[Category: PBG]]

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[[Category: tim barrel]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:17:50 2008''

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@@ Line 4: / Line 4: @@
 ==Overview==
-The X-ray structure of yeast 5-aminolaevulinic acid dehydratase, in which, the catalytic site of the enzyme is complexed with a putative cyclic, intermediate composed of both substrate moieties, has been solved at 0.16, nm (1.6 A) resolution. The cyclic intermediate is bound covalently to, Lys(263) with the amino group of the aminomethyl side chain ligated to the, active-site zinc ion in a position normally occupied by a catalytic, hydroxide ion. The cyclic intermediate is catalytically competent, as, shown by its turnover in the presence of added substrate to form, porphobilinogen. The findings, combined with those of previous studies, are consistent with a catalytic mechanism in which the C-C bond linking, both substrates in the intermediate is formed before the C-N bond.
+The X-ray structure of yeast 5-aminolaevulinic acid dehydratase, in which the catalytic site of the enzyme is complexed with a putative cyclic intermediate composed of both substrate moieties, has been solved at 0.16 nm (1.6 A) resolution. The cyclic intermediate is bound covalently to Lys(263) with the amino group of the aminomethyl side chain ligated to the active-site zinc ion in a position normally occupied by a catalytic hydroxide ion. The cyclic intermediate is catalytically competent, as shown by its turnover in the presence of added substrate to form porphobilinogen. The findings, combined with those of previous studies, are consistent with a catalytic mechanism in which the C-C bond linking both substrates in the intermediate is formed before the C-N bond.
 ==About this Structure==
@@ Line 14: / Line 14: @@
 [[Category: Saccharomyces cerevisiae]]
 [[Category: Single protein]]
-[[Category: Brindley, A.A.]]
+[[Category: Brindley, A A.]]
 [[Category: Butler, D.]]
 [[Category: Coates, L.]]
-[[Category: Cooper, J.B.]]
+[[Category: Cooper, J B.]]
-[[Category: Erskine, P.T.]]
+[[Category: Erskine, P T.]]
-[[Category: Shoolingin-Jordan, P.M.]]
+[[Category: Shoolingin-Jordan, P M.]]
-[[Category: Warren, M.J.]]
+[[Category: Warren, M J.]]
-[[Category: Wood, S.P.]]
+[[Category: Wood, S P.]]
-[[Category: Youell, J.H.]]
+[[Category: Youell, J H.]]
 [[Category: BME]]
 [[Category: PBG]]
@@ Line 32: / Line 32: @@
 [[Category: tim barrel]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb  3 09:58:14 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:17:50 2008''