1ojl: Difference between revisions

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==Overview==

The sigma(54)-dependent transcription in bacteria is associated with, various stress and growth conditions. Activators of the sigma(54) protein, contain a central domain belonging to the AAA+ superfamily of ATPases, members of which function in diverse cellular processes in both, prokaryotic and eukaryotic cells. We describe the X-ray structure of an, N-terminal domain deletion of the ZraR protein from Salmonella, typhimurium, which is a homologue of the general nitrogen regulatory, protein NtrC, at 3A resolution. The structure reveals a hexameric ring, that is typical for AAA+ containing proteins but which differs from the, heptameric ring found in the crystal structure of the AAA+ domain of NtrC1, from Aquifex aeolicus. The dimerisation interface between DNA-binding, domains observed in the crystal structure suggests that dodecamers, rather, than hexamers, might be the functionally important oligomer.

The sigma(54)-dependent transcription in bacteria is associated with various stress and growth conditions. Activators of the sigma(54) protein contain a central domain belonging to the AAA+ superfamily of ATPases, members of which function in diverse cellular processes in both prokaryotic and eukaryotic cells. We describe the X-ray structure of an N-terminal domain deletion of the ZraR protein from Salmonella typhimurium, which is a homologue of the general nitrogen regulatory protein NtrC, at 3A resolution. The structure reveals a hexameric ring that is typical for AAA+ containing proteins but which differs from the heptameric ring found in the crystal structure of the AAA+ domain of NtrC1 from Aquifex aeolicus. The dimerisation interface between DNA-binding domains observed in the crystal structure suggests that dodecamers, rather than hexamers, might be the functionally important oligomer.

==About this Structure==

Line 14:

[[Category: Single protein]]

[[Category: Sallai, L.]]

[[Category: Tucker, P.A.]]

[[Category: Tucker, P A.]]

[[Category: ATP]]

[[Category: PO4]]

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[[Category: two component system]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:18:30 2008''

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@@ Line 4: / Line 4: @@
 ==Overview==
-The sigma(54)-dependent transcription in bacteria is associated with, various stress and growth conditions. Activators of the sigma(54) protein, contain a central domain belonging to the AAA+ superfamily of ATPases, members of which function in diverse cellular processes in both, prokaryotic and eukaryotic cells. We describe the X-ray structure of an, N-terminal domain deletion of the ZraR protein from Salmonella, typhimurium, which is a homologue of the general nitrogen regulatory, protein NtrC, at 3A resolution. The structure reveals a hexameric ring, that is typical for AAA+ containing proteins but which differs from the, heptameric ring found in the crystal structure of the AAA+ domain of NtrC1, from Aquifex aeolicus. The dimerisation interface between DNA-binding, domains observed in the crystal structure suggests that dodecamers, rather, than hexamers, might be the functionally important oligomer.
+The sigma(54)-dependent transcription in bacteria is associated with various stress and growth conditions. Activators of the sigma(54) protein contain a central domain belonging to the AAA+ superfamily of ATPases, members of which function in diverse cellular processes in both prokaryotic and eukaryotic cells. We describe the X-ray structure of an N-terminal domain deletion of the ZraR protein from Salmonella typhimurium, which is a homologue of the general nitrogen regulatory protein NtrC, at 3A resolution. The structure reveals a hexameric ring that is typical for AAA+ containing proteins but which differs from the heptameric ring found in the crystal structure of the AAA+ domain of NtrC1 from Aquifex aeolicus. The dimerisation interface between DNA-binding domains observed in the crystal structure suggests that dodecamers, rather than hexamers, might be the functionally important oligomer.
 ==About this Structure==
@@ Line 14: / Line 14: @@
 [[Category: Single protein]]
 [[Category: Sallai, L.]]
-[[Category: Tucker, P.A.]]
+[[Category: Tucker, P A.]]
 [[Category: ATP]]
 [[Category: PO4]]
@@ Line 24: / Line 24: @@
 [[Category: two component system]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb  3 09:58:58 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:18:30 2008''