1oko: Difference between revisions
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==Overview== | ==Overview== | ||
The structure of the tetrameric Pseudomonas aeruginosa lectin I (PA-IL) in | The structure of the tetrameric Pseudomonas aeruginosa lectin I (PA-IL) in complex with galactose and calcium was determined at 1.6 A resolution, and the native protein was solved at 2.4 A resolution. Each monomer adopts a beta-sandwich fold with ligand binding site at the apex. All galactose hydroxyl groups, except O1, are involved in a hydrogen bond network with the protein and O3 and O4 also participate in the co-ordination of the calcium ion. The stereochemistry of calcium galactose binding is reminiscent of that observed in some animal C-type lectins. The structure of the complex provides a framework for future design of anti-bacterial compounds. | ||
==About this Structure== | ==About this Structure== | ||
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[[Category: lectin]] | [[Category: lectin]] | ||
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Revision as of 12:18, 21 February 2008
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CRYSTAL STRUCTURE OF PSEUDOMONAS AERUGINOSA LECTIN 1 COMPLEXED WITH GALACTOSE AT 1.6 A RESOLUTION
Overview
The structure of the tetrameric Pseudomonas aeruginosa lectin I (PA-IL) in complex with galactose and calcium was determined at 1.6 A resolution, and the native protein was solved at 2.4 A resolution. Each monomer adopts a beta-sandwich fold with ligand binding site at the apex. All galactose hydroxyl groups, except O1, are involved in a hydrogen bond network with the protein and O3 and O4 also participate in the co-ordination of the calcium ion. The stereochemistry of calcium galactose binding is reminiscent of that observed in some animal C-type lectins. The structure of the complex provides a framework for future design of anti-bacterial compounds.
About this Structure
1OKO is a Single protein structure of sequence from Pseudomonas aeruginosa with GAL, CA, SO4 and MPD as ligands. Known structural/functional Site: AC1. Full crystallographic information is available from OCA.
Reference
Structural basis of calcium and galactose recognition by the lectin PA-IL of Pseudomonas aeruginosa., Cioci G, Mitchell EP, Gautier C, Wimmerova M, Sudakevitz D, Perez S, Gilboa-Garber N, Imberty A, FEBS Lett. 2003 Dec 4;555(2):297-301. PMID:14644431
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