1uvm: Difference between revisions

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==Overview==

The RNA-dependent RNA polymerase of bacteriophage phi6 transcribes mRNA, from the three segments of the dsRNA viral genome. We have cocrystallized, RNA oligonucleotides with the polymerase, revealing the mode of binding of, RNA templates. This binding is somewhat different from that previously, seen for DNA oligomers, leading to additional RNA-protein hydrogen bonds, consistent with a preference for RNA. Activation of the RNA/polymerase, complex by the addition of substrate and Mg2+ initiates a single round of, reaction within the crystal to form a dead-end complex that partially, collapses within the enzyme active site. By replacing Mg2+ with Ca2+, we, have been able to capture the inhibited complex which shows distortion, that explains the structural basis for the inhibition of such polymerases, by Ca2+.

The RNA-dependent RNA polymerase of bacteriophage phi6 transcribes mRNA from the three segments of the dsRNA viral genome. We have cocrystallized RNA oligonucleotides with the polymerase, revealing the mode of binding of RNA templates. This binding is somewhat different from that previously seen for DNA oligomers, leading to additional RNA-protein hydrogen bonds, consistent with a preference for RNA. Activation of the RNA/polymerase complex by the addition of substrate and Mg2+ initiates a single round of reaction within the crystal to form a dead-end complex that partially collapses within the enzyme active site. By replacing Mg2+ with Ca2+, we have been able to capture the inhibited complex which shows distortion that explains the structural basis for the inhibition of such polymerases by Ca2+.

==About this Structure==

Line 14:

[[Category: Bamford, D.]]

[[Category: Butcher, S.]]

[[Category: Grimes, J.M.]]

[[Category: Grimes, J M.]]

[[Category: Makeyev, E.V.]]

[[Category: Makeyev, E V.]]

[[Category: Salgado, P.S.]]

[[Category: Salgado, P S.]]

[[Category: Stuart, D.I.]]

[[Category: Stuart, D I.]]

[[Category: MN]]

[[Category: rna-dependent rna polymerase complex with 5nt rna oligonucleotide - conformation a]]

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@@ Line 4: / Line 4: @@
 ==Overview==
-The RNA-dependent RNA polymerase of bacteriophage phi6 transcribes mRNA, from the three segments of the dsRNA viral genome. We have cocrystallized, RNA oligonucleotides with the polymerase, revealing the mode of binding of, RNA templates. This binding is somewhat different from that previously, seen for DNA oligomers, leading to additional RNA-protein hydrogen bonds, consistent with a preference for RNA. Activation of the RNA/polymerase, complex by the addition of substrate and Mg2+ initiates a single round of, reaction within the crystal to form a dead-end complex that partially, collapses within the enzyme active site. By replacing Mg2+ with Ca2+, we, have been able to capture the inhibited complex which shows distortion, that explains the structural basis for the inhibition of such polymerases, by Ca2+.
+The RNA-dependent RNA polymerase of bacteriophage phi6 transcribes mRNA from the three segments of the dsRNA viral genome. We have cocrystallized RNA oligonucleotides with the polymerase, revealing the mode of binding of RNA templates. This binding is somewhat different from that previously seen for DNA oligomers, leading to additional RNA-protein hydrogen bonds, consistent with a preference for RNA. Activation of the RNA/polymerase complex by the addition of substrate and Mg2+ initiates a single round of reaction within the crystal to form a dead-end complex that partially collapses within the enzyme active site. By replacing Mg2+ with Ca2+, we have been able to capture the inhibited complex which shows distortion that explains the structural basis for the inhibition of such polymerases by Ca2+.
 ==About this Structure==
@@ Line 14: / Line 14: @@
 [[Category: Bamford, D.]]
 [[Category: Butcher, S.]]
-[[Category: Grimes, J.M.]]
+[[Category: Grimes, J M.]]
-[[Category: Makeyev, E.V.]]
+[[Category: Makeyev, E V.]]
-[[Category: Salgado, P.S.]]
+[[Category: Salgado, P S.]]
-[[Category: Stuart, D.I.]]
+[[Category: Stuart, D I.]]
 [[Category: MN]]
 [[Category: rna-dependent rna polymerase complex with 5nt rna oligonucleotide - conformation a]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb  3 10:08:25 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:28:45 2008''