1uvp: Difference between revisions

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==Overview==

Two series of complete and highly redundant data sets were collected at, wavelengths of 1.00 and 2.00 Angstroms on a cadmium derivative of porcine, pancreatic elastase (PPE). Radiation damage to the sample was evaluated, qualitatively by inspecting consecutive difference electron density maps, during the course of the experiment. The nature of the radiation damage, was found to be identical at both wavelengths and was localized primarily, at the four disulfide bridges of PPE, the cadmium site and the two, methionine residues. For a quantitative examination of the radiation, damage, the decrease in the peak height of the cadmium ion in various, electron density maps was exploited. Again, no significant difference in, radiation damage between the two wavelengths was observed. This can be, rationalized by considering the wavelength dependencies of the number of, diffracted photons versus the number of absorbed photons and the energy, deposited in the crystal by the latter.

Two series of complete and highly redundant data sets were collected at wavelengths of 1.00 and 2.00 Angstroms on a cadmium derivative of porcine pancreatic elastase (PPE). Radiation damage to the sample was evaluated qualitatively by inspecting consecutive difference electron density maps during the course of the experiment. The nature of the radiation damage was found to be identical at both wavelengths and was localized primarily at the four disulfide bridges of PPE, the cadmium site and the two methionine residues. For a quantitative examination of the radiation damage, the decrease in the peak height of the cadmium ion in various electron density maps was exploited. Again, no significant difference in radiation damage between the two wavelengths was observed. This can be rationalized by considering the wavelength dependencies of the number of diffracted photons versus the number of absorbed photons and the energy deposited in the crystal by the latter.

==About this Structure==

Line 16:

[[Category: Mueller-Dieckmann, C.]]

[[Category: Panjikar, S.]]

[[Category: Tucker, P.A.]]

[[Category: Tucker, P A.]]

[[Category: Weiss, M.S.]]

[[Category: Weiss, M S.]]

[[Category: ACT]]

[[Category: CD]]

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[[Category: serine protease]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:28:47 2008''

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@@ Line 4: / Line 4: @@
 ==Overview==
-Two series of complete and highly redundant data sets were collected at, wavelengths of 1.00 and 2.00 Angstroms on a cadmium derivative of porcine, pancreatic elastase (PPE). Radiation damage to the sample was evaluated, qualitatively by inspecting consecutive difference electron density maps, during the course of the experiment. The nature of the radiation damage, was found to be identical at both wavelengths and was localized primarily, at the four disulfide bridges of PPE, the cadmium site and the two, methionine residues. For a quantitative examination of the radiation, damage, the decrease in the peak height of the cadmium ion in various, electron density maps was exploited. Again, no significant difference in, radiation damage between the two wavelengths was observed. This can be, rationalized by considering the wavelength dependencies of the number of, diffracted photons versus the number of absorbed photons and the energy, deposited in the crystal by the latter.
+Two series of complete and highly redundant data sets were collected at wavelengths of 1.00 and 2.00 Angstroms on a cadmium derivative of porcine pancreatic elastase (PPE). Radiation damage to the sample was evaluated qualitatively by inspecting consecutive difference electron density maps during the course of the experiment. The nature of the radiation damage was found to be identical at both wavelengths and was localized primarily at the four disulfide bridges of PPE, the cadmium site and the two methionine residues. For a quantitative examination of the radiation damage, the decrease in the peak height of the cadmium ion in various electron density maps was exploited. Again, no significant difference in radiation damage between the two wavelengths was observed. This can be rationalized by considering the wavelength dependencies of the number of diffracted photons versus the number of absorbed photons and the energy deposited in the crystal by the latter.
 ==About this Structure==
@@ Line 16: / Line 16: @@
 [[Category: Mueller-Dieckmann, C.]]
 [[Category: Panjikar, S.]]
-[[Category: Tucker, P.A.]]
+[[Category: Tucker, P A.]]
-[[Category: Weiss, M.S.]]
+[[Category: Weiss, M S.]]
 [[Category: ACT]]
 [[Category: CD]]
@@ Line 27: / Line 27: @@
 [[Category: serine protease]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb  3 10:08:27 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:28:47 2008''