2bsw: Difference between revisions
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Revision as of 14:42, 30 October 2007
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CRYSTAL STRUCTURE OF A GLYPHOSATE-N-ACETYLTRANSFERASE OBTAINED BY DNA SHUFFLING.
Overview
The success of structural studies performed on an individual target in, small scale or on many targets in the system-wide scale of structural, genomics depends critically on three parameters: (i) obtaining an, expression system capable of producing large quantities of the, macromolecule(s) of interest, (ii) purifying this material in soluble, form, and (iii) obtaining diffraction-quality crystals suitable for x-ray, analysis. The attrition rate caused by these constraints is often quite, high. Here, we present a strategy that addresses each of these three, parameters simultaneously. Using DNA shuffling to introduce functional, sequence variability into a protein of interest, we screened crude lysate, supernatants for soluble variants that retain enzymatic activity., Crystallization trials ... [(full description)]
About this Structure
2BSW is a [Single protein] structure of sequence from [[1]] with SO4, CAO and GOL as [ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
DNA shuffling as a tool for protein crystallization., Keenan RJ, Siehl DL, Gorton R, Castle LA, Proc Natl Acad Sci U S A. 2005 Jun 21;102(25):8887-92. Epub 2005 Jun 10. PMID:15951425 [[Category: ]]
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