1wae: Difference between revisions
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==Overview== | ==Overview== | ||
Copper nitrite reductases contain both an electron-transfer type 1 Cu site | Copper nitrite reductases contain both an electron-transfer type 1 Cu site and a catalytic type 2 Cu site. We have mutated one of the type 2 copper ligating histidines to observe the effect on catalytic turnover. This mutation has created a unique site where Cu is ligated by 2 His Nepsilon2 atoms alone. | ||
==About this Structure== | ==About this Structure== | ||
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[[Category: Achromobacter xylosoxidans]] | [[Category: Achromobacter xylosoxidans]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Transferred entry: 1 | [[Category: Transferred entry: 1 7.2 1]] | ||
[[Category: Antonyuk, S | [[Category: Antonyuk, S V.]] | ||
[[Category: Eady, R | [[Category: Eady, R R.]] | ||
[[Category: Ellis, M | [[Category: Ellis, M J.]] | ||
[[Category: Hasnain, S | [[Category: Hasnain, S S.]] | ||
[[Category: Sawers, G.]] | [[Category: Sawers, G.]] | ||
[[Category: Strange, R | [[Category: Strange, R W.]] | ||
[[Category: CU]] | [[Category: CU]] | ||
[[Category: ZN]] | [[Category: ZN]] | ||
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[[Category: reductase]] | [[Category: reductase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:42:11 2008'' | ||
Revision as of 13:42, 21 February 2008
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CRYSTAL STRUCTURE OF H129V MUTANT OF ALCALIGENES XYLOSOXIDANS NITRITE REDUCTASE
Overview
Copper nitrite reductases contain both an electron-transfer type 1 Cu site and a catalytic type 2 Cu site. We have mutated one of the type 2 copper ligating histidines to observe the effect on catalytic turnover. This mutation has created a unique site where Cu is ligated by 2 His Nepsilon2 atoms alone.
About this Structure
1WAE is a Single protein structure of sequence from Achromobacter xylosoxidans with CU and ZN as ligands. Active as Transferred entry: 1.7.2.1, with EC number 1.7.99.3 Known structural/functional Site: AC1. Full crystallographic information is available from OCA.
Reference
Observation of an unprecedented Cu Bis-His site: crystal structure of the H129V mutant of nitrite reductase., Ellis MJ, Antonyuk SV, Strange RW, Sawers G, Eady RR, Hasnain SS, Inorg Chem. 2004 Nov 29;43(24):7591-3. PMID:15554622
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