User:Mitchell Long/Sandbox 1: Difference between revisions

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==Mechanism of Bio luminescence==
==Mechanism of Bio luminescence==
Luciferase found in ''''Italic tex<StructureSection load='3fgc' size='500' side='right' caption='Structure of Bacterial Luciferase and FMN complex from ''V. harveyi'' (PDB entry [[3fgc]])' scene=''>
Luciferase found in ''''Vibrio harveyi'''' is a heterodimer that is composed of a catalytic &#945; subunit and a homologous but noncatalytic beta subunit. The catalytic  
 
==Introduction==
Luciferases are a class of enzymes that catalyze the oxidation of a long chain aliphatic aldehydes.  This reaction results in the formation of a carboxylic acid and the emission of photons in the form of blue-green light. 
 
 
 
 
</StructureSection>
 
 
{{STRUCTURE_3fgc|  PDB=3fgc  |  SCENE=  }}
 
 
 
==Mechanism of Bio luminescence==
Luciferase found in ''''Vibrio harveyi'''' is a heterodimeric flavin monoxygenase that is composed of a catalytic &#945; subunit and a homologous but noncatalytic &#946; subunit. The catalytic &#945; subunit is bound to a Flavin Mononucleotide cofactor. Bioluminescense is achieved by a two step reaction between




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Active Site
Active Site


Mobile Loop-The mobile loop of luciferase is thought to be responsible for protecting reaction intermediates and preventing the entry of bulk solvent into the active center once the flavin substrate is bound. 
Mobile Loop
 
(&#946;/&#945;)<SUP>8</SUP> Barrel
(&#946;/&#945;)&sub8; Barrel-
 
Interface between Alpha and Beta Subunits
Interface between Alpha and Beta Subunits


==Applications In Biotechnology==
==Applications In Biotechnology==

Revision as of 03:16, 17 November 2011

Structure of Bacterial Luciferase and FMN complex from

Drag the structure with the mouse to rotate


Drag the structure with the mouse to rotate
3fgc, resolution 2.30Å (default scene)
Ligands: FMN, PO4, SO4
Gene: luxA (Vibrio harveyi), luxB (Vibrio harveyi)
Activity: Alkanal monooxygenase (FMN-linked), with EC number 1.14.14.3
Related: 1luc, 1brl
Resources: FirstGlance, OCA, RCSB, PDBsum
Coordinates: save as pdb, mmCIF, xml




Mechanism of Bio luminescence

Luciferase found in 'Vibrio harveyi' is a heterodimer that is composed of a catalytic α subunit and a homologous but noncatalytic beta subunit. The catalytic


Structural Motifs

Active Site

Mobile Loop (β/α)8 Barrel Interface between Alpha and Beta Subunits

Applications In Biotechnology

The luciferase assay

Quorum Sensing

Quorum sensing is a population density dependent form gene expression found in many bacteria.



References

Proteopedia Page Contributors and Editors (what is this?)

Mitchell Long

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