User:Iris To/Retinoblastoma Protein Regulation: Difference between revisions
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==Regulation of Rb== | ==Regulation of Rb== | ||
{{STRUCTURE_3n5u| PDB=3n5u | SCENE= }} | {{STRUCTURE_3n5u| PDB=3n5u | SCENE= }} | ||
From previous studies, it has been determined that the phosphorylation state and activity of Rb are controlled by a balance of kinase and phosphatase activity | From previous studies, it has been determined that the phosphorylation state and activity of Rb are controlled by a balance of kinase and phosphatase activity, in which Cdks phosphorylate Rb from late G1 to mitosis and the enzyme protein phosphatase 1 (PP1c) dephosphorylates Rb for mitotic exit<ref name=Hirschi>PMID: 20694007</ref>. An enzyme-docking site for PP1c was determined to overlap with the docking site for Cdks, more specifically the RxL Cyclin binding site<ref name=Lowe>PMID:12501191</ref> that brings Cdk-Cyclins to Rb, through a crystal structure; this crystal structure initiated studies on phosphatase and kinase competition for the docking site. Studies determined a biochemical mechanism where directly competing kinases and phosphatase activity regulates Rb phosphorylation and activity, but there has not been an established study that defined a mechanism that controlled the outcome of the competition between each enzyme<ref name=Hirschi>PMID: 20694007</ref>. | ||
==Structure== | ==Structure== | ||
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==Importance== | ==Importance== | ||
==3D | ==3D Structures== | ||
[[1o9k]] with E2F<br /> | [[1o9k]] with E2F<br /> | ||
[[1h25]] with Cdk2/Cyclin A<br /> | [[1h25]] with Cdk2/Cyclin A<br /> | ||
