ToxT: Difference between revisions
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==Introduction== | ==Introduction== | ||
<b>ToxT</b> is a molecule at the end of a transcriptional cascade that autoregulates the transcription of the primary virulence factors of <i>Vibrio cholerae</i>[http://http://en.wikipedia.org/wiki/Vibrio_cholerae] and itself. These two factors, cholera toxin (CT)[http://http://en.wikipedia.org/wiki/Cholera_toxin] and the toxin co-regulated pilus (TCP), are instrumental in causing the disease <b>cholera</b>[http://http://en.wikipedia.org/wiki/Cholera]. This is an intestinal infection resulting in massive water loss in the affected individual, causing extreme dehydration.[http://http://en.wikipedia.org/wiki/Cholera] | <b>ToxT</b> is a molecule at the end of a transcriptional cascade that autoregulates the transcription of the primary virulence factors of <i>Vibrio cholerae</i>[http://http://en.wikipedia.org/wiki/Vibrio_cholerae] and itself. These two factors, cholera toxin (CT)[http://http://en.wikipedia.org/wiki/Cholera_toxin] and the toxin co-regulated pilus (TCP), are instrumental in causing the disease <b>cholera</b>[http://http://en.wikipedia.org/wiki/Cholera]. This is an intestinal infection resulting in massive water loss in the affected individual, causing extreme dehydration.[http://http://en.wikipedia.org/wiki/Cholera] | ||
==Ligand== | |||
In this resolved structure, <scene name='ToxT_Transcriptional_Regulator_in_Vibrio_cholerae/Pam/2'><i>cis</i>-palmitoleate</scene>[http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl?pdbcode=3gbg&template=ligands.html&l=1.1] is present and bound in the beta sheet barrel (further discussed below). This unsaturated fatty acid reduces virulence expression in <i>Vibrio cholerae</i>. | |||
==Structural Features== | ==Structural Features== | ||
ToxT belongs to a family of transcriptional regulators headed by and known as AraC.<ref name="structure">PMID: 20133655</ref> The AraC family is characterized by a 100 amino acid region of sequence similarity that forms a DNA-binding domain with two helix-turn-helix motifs. <ref name="arac">PMID: 11282467</ref> The two HTH regions are linked by a very polar alpha helix. The overall domain is composed of seven alpha helices.<ref name="structure">PMID: 20133655</ref> | ToxT belongs to a family of transcriptional regulators headed by and known as AraC.<ref name="structure">PMID: 20133655</ref> The AraC family is characterized by a 100 amino acid region of sequence similarity that forms a DNA-binding domain with two helix-turn-helix motifs. <ref name="arac">PMID: 11282467</ref> The two HTH regions are linked by a very polar alpha helix. The overall domain is composed of seven alpha helices.<ref name="structure">PMID: 20133655</ref> | ||
