ToxT: Difference between revisions
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The crystal structure of ToxT is resolved in monomeric form, after isolation from <i>Vibrio cholerae</i> strain O395.<ref name="structure">PMID: 20133655</ref> | The crystal structure of ToxT is resolved in monomeric form, after isolation from <i>Vibrio cholerae</i> strain O395.<ref name="structure">PMID: 20133655</ref> | ||
==Introduction== | ==Introduction== | ||
<b>ToxT</b> is a molecule at the end of a transcriptional cascade that autoregulates the transcription of the primary virulence factors of <i>Vibrio cholerae</i>[ | <b>ToxT</b> is a molecule at the end of a transcriptional cascade that autoregulates the transcription of the primary virulence factors of <i>Vibrio cholerae</i>[http://en.wikipedia.org/wiki/Vibrio_cholerae] and itself. These two factors, cholera toxin (CT)[http://http://en.wikipedia.org/wiki/Cholera_toxin] and the toxin co-regulated pilus (TCP), are instrumental in causing the disease <b>cholera</b>[http://en.wikipedia.org/wiki/Cholera]. This is an intestinal infection resulting in massive water loss in the affected individual, causing extreme dehydration.[http://en.wikipedia.org/wiki/Cholera] | ||
==Ligand== | ==Ligand== | ||
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<scene name='ToxT_Transcriptional_Regulator_in_Vibrio_cholerae/Barrel/1'>A nine-stranded beta sheet sandwich</scene> contains a binding pocket that contains a ligand: <i>cis</i>-palmitoleate,<!--insert scene here!--> <ref name="structure">PMID: 20133655</ref> which appears to have a negative effect on virulence when present in vitro. This unsaturated fatty acid, like other UFAs,[ | <scene name='ToxT_Transcriptional_Regulator_in_Vibrio_cholerae/Barrel/1'>A nine-stranded beta sheet sandwich</scene> contains a binding pocket that contains a ligand: <i>cis</i>-palmitoleate,<!--insert scene here!--> <ref name="structure">PMID: 20133655</ref> which appears to have a negative effect on virulence when present in vitro. This unsaturated fatty acid, like other UFAs,[http://en.wikipedia.org/wiki/Fatty_acid#Unsaturated_fatty_acids] tend to inhibit genes under the control of ToxT. | ||
Specifically, the <i>cis</i>-palmitoleate appears to bind directly to ToxT, change its conformation, and thus lower the ability to bind DNA and form dimers.<ref name="structure">PMID: 20133655</ref> | Specifically, the <i>cis</i>-palmitoleate appears to bind directly to ToxT, change its conformation, and thus lower the ability to bind DNA and form dimers.<ref name="structure">PMID: 20133655</ref> | ||
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