ToxT: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 3: | Line 3: | ||
<b>ToxT</b> is a molecule at the end of a transcriptional cascade that autoregulates the transcription of the primary virulence factors of <i>Vibrio cholerae</i>[http://en.wikipedia.org/wiki/Vibrio_cholerae] and itself. These two factors, cholera toxin (CT)[http://http://en.wikipedia.org/wiki/Cholera_toxin] and the toxin co-regulated pilus (TCP), are instrumental in causing the disease <b>cholera</b>[http://en.wikipedia.org/wiki/Cholera]. This is an intestinal infection resulting in massive water loss in the affected individual, causing extreme dehydration.[http://en.wikipedia.org/wiki/Cholera] | <b>ToxT</b> is a molecule at the end of a transcriptional cascade that autoregulates the transcription of the primary virulence factors of <i>Vibrio cholerae</i>[http://en.wikipedia.org/wiki/Vibrio_cholerae] and itself. These two factors, cholera toxin (CT)[http://http://en.wikipedia.org/wiki/Cholera_toxin] and the toxin co-regulated pilus (TCP), are instrumental in causing the disease <b>cholera</b>[http://en.wikipedia.org/wiki/Cholera]. This is an intestinal infection resulting in massive water loss in the affected individual, causing extreme dehydration.[http://en.wikipedia.org/wiki/Cholera] | ||
<StructureSection load='3gbg' color='cpk' size='500' frame='true' align='right' caption='ToxT, 1.9 Angstrom resolution crystal structure' > | <StructureSection load='3gbg' color='cpk' size='500' frame='true' align='right' caption='ToxT, 1.9 Angstrom resolution crystal structure' > | ||
==Ligand== | ==Ligand== | ||
In this resolved structure, <scene name='ToxT_Transcriptional_Regulator_in_Vibrio_cholerae/Pam/2'>cis-palmitoleate</scene>[http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl?pdbcode=3gbg&template=ligands.html&l=1.1] is present and bound in the beta sheet barrel (further discussed below). This unsaturated fatty acid reduces virulence expression in <i>Vibrio cholerae</i>. | In this resolved structure, <scene name='ToxT_Transcriptional_Regulator_in_Vibrio_cholerae/Pam/2'>cis-palmitoleate</scene>[http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl?pdbcode=3gbg&template=ligands.html&l=1.1] is present and bound in the beta sheet barrel (further discussed below). This unsaturated fatty acid reduces virulence expression in <i>Vibrio cholerae</i>. | ||
| Line 15: | Line 14: | ||
<br/> | <br/> | ||
<br/> | <br/> | ||
Though the structure shown is a monomer with two overall domains (N-terminal and C-terminal), ToxT tends to form a dimer. The preferred state of ToxT varies between promoters, but binding to the <i>ctx</i> promoter to generate cholera toxin appears to be possible only in the dimer form.<ref name="virstatin">PMID:17283330</ref>ToxT binds to thirteen base pair sequences (can be single, direct, or inverted repeats) called toxboxes in order to activate their respective promoters.[http://www.sigwiki.info/wiki/Signature:ToxBox] | Though the structure shown is a monomer with two overall domains (N-terminal and C-terminal), ToxT tends to form a dimer.<ref name="dimerization">PMID: 21415495 | ||
</ref> The preferred state of ToxT varies between promoters, but binding to the <i>ctx</i> promoter to generate cholera toxin appears to be possible only in the dimer form.<ref name="virstatin">PMID:17283330</ref>ToxT binds to thirteen base pair sequences (can be single, direct, or inverted repeats) called toxboxes in order to activate their respective promoters.[http://www.sigwiki.info/wiki/Signature:ToxBox] | |||
</StructureSection> | </StructureSection> | ||
==References== | ==References== | ||
<references/> | <references/> | ||
