2boj: Difference between revisions
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==Overview== | ==Overview== | ||
The lectin from Pseudomonas aeruginosa (PA-IIL) is involved in host | The lectin from Pseudomonas aeruginosa (PA-IIL) is involved in host recognition and biofilm formation. Lectin not only displays an unusually high affinity for fucose but also binds to L-fucose, L-galactose and D-arabinose that differ only by the group at position 5 of the sugar ring. Isothermal calorimetry experiments provided precise determination of affinity for the three methyl-glycosides and revealed a large enthalpy contribution. The crystal structures of the complexes of PA-IIL with L-galactose and Met-beta-D-arabinoside have been determined and compared with the PA-IIL/fucose complex described previously. A combination of the structures and thermodynamics provided clues for the role of the hydrophobic group in affinity. | ||
==About this Structure== | ==About this Structure== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Imberty, A.]] | [[Category: Imberty, A.]] | ||
[[Category: Mitchell, E | [[Category: Mitchell, E P.]] | ||
[[Category: Sabin, C | [[Category: Sabin, C D.]] | ||
[[Category: Wimmerova, M.]] | [[Category: Wimmerova, M.]] | ||
[[Category: ARW]] | [[Category: ARW]] | ||
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[[Category: lewis a]] | [[Category: lewis a]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:40:01 2008'' | ||
Revision as of 14:40, 21 February 2008
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CRYSTAL STRUCTURE OF PSEUDOMONAS AERUGINOSA LECTIN (PA-IIL) COMPLEXED WITH METHYL-B-D-ARABINOPYRANOSIDE
Overview
The lectin from Pseudomonas aeruginosa (PA-IIL) is involved in host recognition and biofilm formation. Lectin not only displays an unusually high affinity for fucose but also binds to L-fucose, L-galactose and D-arabinose that differ only by the group at position 5 of the sugar ring. Isothermal calorimetry experiments provided precise determination of affinity for the three methyl-glycosides and revealed a large enthalpy contribution. The crystal structures of the complexes of PA-IIL with L-galactose and Met-beta-D-arabinoside have been determined and compared with the PA-IIL/fucose complex described previously. A combination of the structures and thermodynamics provided clues for the role of the hydrophobic group in affinity.
About this Structure
2BOJ is a Single protein structure of sequence from Pseudomonas aeruginosa with CA, SO4 and ARW as ligands. Known structural/functional Site: AC1. Full crystallographic information is available from OCA.
Reference
Binding of different monosaccharides by lectin PA-IIL from Pseudomonas aeruginosa: thermodynamics data correlated with X-ray structures., Sabin C, Mitchell EP, Pokorna M, Gautier C, Utille JP, Wimmerova M, Imberty A, FEBS Lett. 2006 Feb 6;580(3):982-7. Epub 2006 Jan 19. PMID:16438968
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