3ts2: Difference between revisions

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[[~~Image~~:~~3ts2~~.~~png~~|~~left~~|~~200px~~]]

==Mouse Lin28A in complex with let-7g microRNA pre-element==

== Structural highlights ==

<table><tr><td colspan='2'>[[3ts2]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TS2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TS2 FirstGlance]. <br>

</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>

<tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=GMP:GUANOSINE'>GMP</scene></td></tr>

<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Lin28, Lin28a, Tex17 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>

<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ts2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ts2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ts2 RCSB], [http://www.ebi.ac.uk/pdbsum/3ts2 PDBsum]</span></td></tr>

<table>

== Publication Abstract from PubMed ==

MicroRNAs (miRNAs) are small noncoding RNA molecules that regulate gene expression. Among these, members of the let-7 miRNA family control many cell-fate determination genes to influence pluripotency, differentiation, and transformation. Lin28 is a specific, posttranscriptional inhibitor of let-7 biogenesis. We report crystal structures of mouse Lin28 in complex with sequences from let-7d, let-7-f1, and let-7g precursors. The two folded domains of Lin28 recognize two distinct regions of the RNA and are sufficient for inhibition of let-7 in vivo. We also show by NMR spectroscopy that the linker connecting the two folded domains is flexible, accommodating Lin28 binding to diverse let-7 family members. Protein-RNA complex formation imposes specific conformations on both components that could affect downstream recognition by other processing factors. Our data provide a molecular explanation for Lin28 specificity and a model for how it regulates let-7.

<!--

Molecular Basis for Interaction of let-7 MicroRNAs with Lin28.,Nam Y, Chen C, Gregory RI, Chou JJ, Sliz P Cell. 2011 Nov 23;147(5):1080-91. Epub 2011 Nov 10. PMID:22078496<ref>PMID:22078496</ref>

~~The line below this paragraph~~, ~~containing "STRUCTURE_3ts2"~~, ~~creates the "Structure Box" on the page~~.

~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)

~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~

~~or leave the SCENE parameter empty for the default display~~.

-->

~~{{STRUCTURE_3ts2| PDB=3ts2 | SCENE= }}~~

~~===Mouse Lin28A in complex with let-7g microRNA pre-element===~~

From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>

</div>

== References ==

~~<!--~~

~~The line below this paragraph~~, ~~{{ABSTRACT_PUBMED_22078496}}, adds the Publication Abstract to~~ the ~~page~~

__TOC__

~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 22078496 is the PubMed ID number~~.

</StructureSection>

-->

~~{{ABSTRACT_PUBMED_22078496}}~~

~~==About this Structure==~~

~~[[3ts2]] is a 4 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TS2 OCA].~~

==~~Reference~~==

<~~ref group="xtra">PMID:022078496<~~/~~ref~~><~~references group="xtra"~~/>

[[Category: Mus musculus]]

[[Category: Nam, Y.]]

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@@ Line 1: / Line 1: @@
-[[Image:3ts2.png|left|200px]]
+==Mouse Lin28A in complex with let-7g microRNA pre-element==
+<StructureSection load='3ts2' size='340' side='right' caption='[[3ts2]], [[Resolution|resolution]] 2.01&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[3ts2]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TS2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TS2 FirstGlance]. <br>
+</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
+<tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=GMP:GUANOSINE'>GMP</scene></td></tr>
+<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Lin28, Lin28a, Tex17 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>
+<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ts2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ts2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ts2 RCSB], [http://www.ebi.ac.uk/pdbsum/3ts2 PDBsum]</span></td></tr>
+<table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+MicroRNAs (miRNAs) are small noncoding RNA molecules that regulate gene expression. Among these, members of the let-7 miRNA family control many cell-fate determination genes to influence pluripotency, differentiation, and transformation. Lin28 is a specific, posttranscriptional inhibitor of let-7 biogenesis. We report crystal structures of mouse Lin28 in complex with sequences from let-7d, let-7-f1, and let-7g precursors. The two folded domains of Lin28 recognize two distinct regions of the RNA and are sufficient for inhibition of let-7 in vivo. We also show by NMR spectroscopy that the linker connecting the two folded domains is flexible, accommodating Lin28 binding to diverse let-7 family members. Protein-RNA complex formation imposes specific conformations on both components that could affect downstream recognition by other processing factors. Our data provide a molecular explanation for Lin28 specificity and a model for how it regulates let-7.
-<!--
+Molecular Basis for Interaction of let-7 MicroRNAs with Lin28.,Nam Y, Chen C, Gregory RI, Chou JJ, Sliz P Cell. 2011 Nov 23;147(5):1080-91. Epub 2011 Nov 10. PMID:22078496<ref>PMID:22078496</ref>
-The line below this paragraph, containing "STRUCTURE_3ts2", creates the "Structure Box" on the page.
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-or leave the SCENE parameter empty for the default display.
--->
-{{STRUCTURE_3ts2|  PDB=3ts2  |  SCENE=  }}
-===Mouse Lin28A in complex with let-7g microRNA pre-element===
+From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
+</div>
+== References ==
-<!--
+<references/>
-The line below this paragraph, {{ABSTRACT_PUBMED_22078496}}, adds the Publication Abstract to the page
+__TOC__
-(as it appears on PubMed at http://www.pubmed.gov), where 22078496 is the PubMed ID number.
+</StructureSection>
--->
-{{ABSTRACT_PUBMED_22078496}}
-==About this Structure==
-[[3ts2]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TS2 OCA].
-==Reference==
-<ref group="xtra">PMID:022078496</ref><references group="xtra"/>
 [[Category: Mus musculus]]
 [[Category: Nam, Y.]]