2bra: Difference between revisions

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==Overview==

During development, neurons are guided to their targets by short- and, long-range attractive and repulsive cues. MICAL, a large multidomain, protein, is required for the combined action of semaphorins and plexins in, axon guidance. Here, we present the structure of the N-terminal region of, MICAL (MICAL(fd)) determined by x-ray diffraction to 2.0 A resolution. The, structure shows that MICAL(fd) is an FAD-containing module structurally, similar to aromatic hydroxylases and amine oxidases. In addition, we, present biochemical data that show that MICAL(fd) is a flavoenzyme that in, the presence of NADPH reduces molecular oxygen to H(2)O(2) (K(m,NAPDH) =, 222 microM; k(cat) = 77 sec(-1)), a molecule with known signaling, properties. We propose that the H(2)O(2) produced by this reaction may be, one of the signaling molecules involved in axon guidance by MICAL.

During development, neurons are guided to their targets by short- and long-range attractive and repulsive cues. MICAL, a large multidomain protein, is required for the combined action of semaphorins and plexins in axon guidance. Here, we present the structure of the N-terminal region of MICAL (MICAL(fd)) determined by x-ray diffraction to 2.0 A resolution. The structure shows that MICAL(fd) is an FAD-containing module structurally similar to aromatic hydroxylases and amine oxidases. In addition, we present biochemical data that show that MICAL(fd) is a flavoenzyme that in the presence of NADPH reduces molecular oxygen to H(2)O(2) (K(m,NAPDH) = 222 microM; k(cat) = 77 sec(-1)), a molecule with known signaling properties. We propose that the H(2)O(2) produced by this reaction may be one of the signaling molecules involved in axon guidance by MICAL.

==About this Structure==

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[[Category: Mus musculus]]

[[Category: Single protein]]

[[Category: Amzel, L.M.]]

[[Category: Amzel, L M.]]

[[Category: Bianchet, M.A.]]

[[Category: Bianchet, M A.]]

[[Category: Gabelli, S.B.]]

[[Category: Gabelli, S B.]]

[[Category: Nadella, M.]]

[[Category: CL]]

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[[Category: zinc]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:40:47 2008''

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@@ Line 4: / Line 4: @@
 ==Overview==
-During development, neurons are guided to their targets by short- and, long-range attractive and repulsive cues. MICAL, a large multidomain, protein, is required for the combined action of semaphorins and plexins in, axon guidance. Here, we present the structure of the N-terminal region of, MICAL (MICAL(fd)) determined by x-ray diffraction to 2.0 A resolution. The, structure shows that MICAL(fd) is an FAD-containing module structurally, similar to aromatic hydroxylases and amine oxidases. In addition, we, present biochemical data that show that MICAL(fd) is a flavoenzyme that in, the presence of NADPH reduces molecular oxygen to H(2)O(2) (K(m,NAPDH) =, 222 microM; k(cat) = 77 sec(-1)), a molecule with known signaling, properties. We propose that the H(2)O(2) produced by this reaction may be, one of the signaling molecules involved in axon guidance by MICAL.
+During development, neurons are guided to their targets by short- and long-range attractive and repulsive cues. MICAL, a large multidomain protein, is required for the combined action of semaphorins and plexins in axon guidance. Here, we present the structure of the N-terminal region of MICAL (MICAL(fd)) determined by x-ray diffraction to 2.0 A resolution. The structure shows that MICAL(fd) is an FAD-containing module structurally similar to aromatic hydroxylases and amine oxidases. In addition, we present biochemical data that show that MICAL(fd) is a flavoenzyme that in the presence of NADPH reduces molecular oxygen to H(2)O(2) (K(m,NAPDH) = 222 microM; k(cat) = 77 sec(-1)), a molecule with known signaling properties. We propose that the H(2)O(2) produced by this reaction may be one of the signaling molecules involved in axon guidance by MICAL.
 ==About this Structure==
@@ Line 13: / Line 13: @@
 [[Category: Mus musculus]]
 [[Category: Single protein]]
-[[Category: Amzel, L.M.]]
+[[Category: Amzel, L M.]]
-[[Category: Bianchet, M.A.]]
+[[Category: Bianchet, M A.]]
-[[Category: Gabelli, S.B.]]
+[[Category: Gabelli, S B.]]
 [[Category: Nadella, M.]]
 [[Category: CL]]
@@ Line 31: / Line 31: @@
 [[Category: zinc]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb  3 10:27:00 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:40:47 2008''