2bu9: Difference between revisions
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==Overview== | ==Overview== | ||
Isopenicillin N synthase (IPNS) is a non-haem iron oxidase that catalyses | Isopenicillin N synthase (IPNS) is a non-haem iron oxidase that catalyses the formation of isopenicillin N from the tripeptide delta-(L-alpha-aminoadipoyl)-L-cysteinyl-D-valine. In this report, we describe the crystal structure of the enzyme with a non-natural L,L,L-tripeptide substrate, delta-(L-alpha-aminoadipoyl)-L-cysteinyl-L-3,3,3,3',3',3'-hexafluorovaline . This structure reveals a strong binding interaction of the tripeptide within the active site and a unique conformation for the non-natural L,L,L-diastereomer. Taken together, these findings provide a possible rationale for the previously observed inhibitory effects of L,L,L-tripeptide substrates on IPNS activity. | ||
==About this Structure== | ==About this Structure== | ||
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[[Category: Isopenicillin-N synthase]] | [[Category: Isopenicillin-N synthase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Adlington, R | [[Category: Adlington, R M.]] | ||
[[Category: Baldwin, J | [[Category: Baldwin, J E.]] | ||
[[Category: Clifton, I | [[Category: Clifton, I J.]] | ||
[[Category: Howard-Jones, A | [[Category: Howard-Jones, A R.]] | ||
[[Category: Rutledge, P | [[Category: Rutledge, P J.]] | ||
[[Category: FE]] | [[Category: FE]] | ||
[[Category: HFV]] | [[Category: HFV]] | ||
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[[Category: penicillin biosynthesis]] | [[Category: penicillin biosynthesis]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:41:47 2008'' | ||
