2buo: Difference between revisions

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==Overview==

Immature HIV particles bud from infected cells after assembly at the, cytoplasmic side of cellular membranes. This assembly is driven by, interactions between Gag polyproteins. Mature particles, each containing a, characteristic conical core, are later generated by proteolytic maturation, of Gag in the virion. The C-terminal domain of the HIV-1 capsid protein, (C-CA) has been shown to contain oligomerization determinants essential, for particle assembly. Here we report the 1.7-A-resolution crystal, structure of C-CA in complex with a peptide capable of inhibiting, immature- and mature-like particle assembly in vitro. The peptide inserts, as an amphipathic alpha-helix into a conserved hydrophobic groove of C-CA, resulting in formation of a compact five-helix bundle with altered dimeric, interactions. This structure thus reveals the details of an allosteric, site in the HIV capsid protein that can be targeted for antiviral therapy.

Immature HIV particles bud from infected cells after assembly at the cytoplasmic side of cellular membranes. This assembly is driven by interactions between Gag polyproteins. Mature particles, each containing a characteristic conical core, are later generated by proteolytic maturation of Gag in the virion. The C-terminal domain of the HIV-1 capsid protein (C-CA) has been shown to contain oligomerization determinants essential for particle assembly. Here we report the 1.7-A-resolution crystal structure of C-CA in complex with a peptide capable of inhibiting immature- and mature-like particle assembly in vitro. The peptide inserts as an amphipathic alpha-helix into a conserved hydrophobic groove of C-CA, resulting in formation of a compact five-helix bundle with altered dimeric interactions. This structure thus reveals the details of an allosteric site in the HIV capsid protein that can be targeted for antiviral therapy.

==About this Structure==

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[[Category: Protein complex]]

[[Category: Duquerroy, S.]]

[[Category: Krausslich, H.G.]]

[[Category: Krausslich, H G.]]

[[Category: Rey, F.A.]]

[[Category: Rey, F A.]]

[[Category: Sticht, J.]]

[[Category: Ternois, F.]]

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[[Category: polyprotein]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:41:48 2008''

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@@ Line 4: / Line 4: @@
 ==Overview==
-Immature HIV particles bud from infected cells after assembly at the, cytoplasmic side of cellular membranes. This assembly is driven by, interactions between Gag polyproteins. Mature particles, each containing a, characteristic conical core, are later generated by proteolytic maturation, of Gag in the virion. The C-terminal domain of the HIV-1 capsid protein, (C-CA) has been shown to contain oligomerization determinants essential, for particle assembly. Here we report the 1.7-A-resolution crystal, structure of C-CA in complex with a peptide capable of inhibiting, immature- and mature-like particle assembly in vitro. The peptide inserts, as an amphipathic alpha-helix into a conserved hydrophobic groove of C-CA, resulting in formation of a compact five-helix bundle with altered dimeric, interactions. This structure thus reveals the details of an allosteric, site in the HIV capsid protein that can be targeted for antiviral therapy.
+Immature HIV particles bud from infected cells after assembly at the cytoplasmic side of cellular membranes. This assembly is driven by interactions between Gag polyproteins. Mature particles, each containing a characteristic conical core, are later generated by proteolytic maturation of Gag in the virion. The C-terminal domain of the HIV-1 capsid protein (C-CA) has been shown to contain oligomerization determinants essential for particle assembly. Here we report the 1.7-A-resolution crystal structure of C-CA in complex with a peptide capable of inhibiting immature- and mature-like particle assembly in vitro. The peptide inserts as an amphipathic alpha-helix into a conserved hydrophobic groove of C-CA, resulting in formation of a compact five-helix bundle with altered dimeric interactions. This structure thus reveals the details of an allosteric site in the HIV capsid protein that can be targeted for antiviral therapy.
 ==About this Structure==
@@ Line 14: / Line 14: @@
 [[Category: Protein complex]]
 [[Category: Duquerroy, S.]]
-[[Category: Krausslich, H.G.]]
+[[Category: Krausslich, H G.]]
-[[Category: Rey, F.A.]]
+[[Category: Rey, F A.]]
 [[Category: Sticht, J.]]
 [[Category: Ternois, F.]]
@@ Line 26: / Line 26: @@
 [[Category: polyprotein]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb  3 10:27:58 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:41:48 2008''