3zyl: Difference between revisions

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[[~~Image~~:~~3zyl~~.~~jpg~~|~~left~~|~~200px~~]]

==STRUCTURE OF A TRUNCATED CALM (PICALM) ANTH DOMAIN==

== Structural highlights ==

<table><tr><td colspan='2'>[[3zyl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZYL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZYL FirstGlance]. <br>

<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zyl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zyl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zyl RCSB], [http://www.ebi.ac.uk/pdbsum/3zyl PDBsum]</span></td></tr>

<table>

== Publication Abstract from PubMed ==

SNAREs provide a large part of the specificity and energy needed for membrane fusion and, to do so, must be localized to their correct membranes. Here, we show that the R-SNAREs VAMP8, VAMP3, and VAMP2, which cycle between the plasma membrane and endosomes, bind directly to the ubiquitously expressed, PtdIns4,5P(2)-binding, endocytic clathrin adaptor CALM/PICALM. X-ray crystallography shows that the N-terminal halves of their SNARE motifs bind the CALM(ANTH) domain as helices in a manner that mimics SNARE complex formation. Mutation of residues in the CALM:SNARE interface inhibits binding in vitro and prevents R-SNARE endocytosis in vivo. Thus, CALM:R-SNARE interactions ensure that R-SNAREs, required for the fusion of endocytic clathrin-coated vesicles with endosomes and also for subsequent postendosomal trafficking, are sorted into endocytic vesicles. CALM's role in directing the endocytosis of small R-SNAREs may provide insight into the association of CALM/PICALM mutations with growth retardation, cognitive defects, and Alzheimer's disease.

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The Molecular Basis for the Endocytosis of Small R-SNAREs by the Clathrin Adaptor CALM.,Miller SE, Sahlender DA, Graham SC, Honing S, Robinson MS, Peden AA, Owen DJ Cell. 2011 Nov 23;147(5):1118-31. PMID:22118466<ref>PMID:22118466</ref>

~~The line below this paragraph~~, ~~containing "STRUCTURE_3zyl"~~, ~~creates the "Structure Box" on the page~~.

~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)

~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~

~~or leave the SCENE parameter empty for the default display~~.

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~~{{STRUCTURE_3zyl| PDB=3zyl | SCENE= }}~~

~~===STRUCTURE OF A TRUNCATED CALM (PICALM) ANTH DOMAIN===~~

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

</div>

== References ==

~~<!--~~

~~The line below this paragraph~~, ~~{{ABSTRACT_PUBMED_22118466}}, adds the Publication Abstract to~~ the ~~page~~

__TOC__

~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 22118466 is the PubMed ID number~~.

</StructureSection>

-->

~~{{ABSTRACT_PUBMED_22118466}}~~

~~==About this Structure==~~

~~[[3zyl]] is a 2 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZYL OCA].~~

==~~Reference~~==

<~~ref group="xtra">PMID:022118466<~~/~~ref~~><~~references group="xtra"~~/>

[[Category: Rattus norvegicus]]

[[Category: Graham, S C.]]

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@@ Line 1: / Line 1: @@
-[[Image:3zyl.jpg|left|200px]]
+==STRUCTURE OF A TRUNCATED CALM (PICALM) ANTH DOMAIN==
+<StructureSection load='3zyl' size='340' side='right' caption='[[3zyl]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[3zyl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZYL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZYL FirstGlance]. <br>
+</td></tr><tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1hg2|1hg2]], [[1hfa|1hfa]], [[1hg5|1hg5]], [[1hf8|1hf8]], [[3zym|3zym]], [[3zyk|3zyk]]</td></tr>
+<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zyl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zyl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zyl RCSB], [http://www.ebi.ac.uk/pdbsum/3zyl PDBsum]</span></td></tr>
+<table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+SNAREs provide a large part of the specificity and energy needed for membrane fusion and, to do so, must be localized to their correct membranes. Here, we show that the R-SNAREs VAMP8, VAMP3, and VAMP2, which cycle between the plasma membrane and endosomes, bind directly to the ubiquitously expressed, PtdIns4,5P(2)-binding, endocytic clathrin adaptor CALM/PICALM. X-ray crystallography shows that the N-terminal halves of their SNARE motifs bind the CALM(ANTH) domain as helices in a manner that mimics SNARE complex formation. Mutation of residues in the CALM:SNARE interface inhibits binding in vitro and prevents R-SNARE endocytosis in vivo. Thus, CALM:R-SNARE interactions ensure that R-SNAREs, required for the fusion of endocytic clathrin-coated vesicles with endosomes and also for subsequent postendosomal trafficking, are sorted into endocytic vesicles. CALM's role in directing the endocytosis of small R-SNAREs may provide insight into the association of CALM/PICALM mutations with growth retardation, cognitive defects, and Alzheimer's disease.
-<!--
+The Molecular Basis for the Endocytosis of Small R-SNAREs by the Clathrin Adaptor CALM.,Miller SE, Sahlender DA, Graham SC, Honing S, Robinson MS, Peden AA, Owen DJ Cell. 2011 Nov 23;147(5):1118-31. PMID:22118466<ref>PMID:22118466</ref>
-The line below this paragraph, containing "STRUCTURE_3zyl", creates the "Structure Box" on the page.
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-or leave the SCENE parameter empty for the default display.
--->
-{{STRUCTURE_3zyl|  PDB=3zyl  |  SCENE=  }}
-===STRUCTURE OF A TRUNCATED CALM (PICALM) ANTH DOMAIN===
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+== References ==
-<!--
+<references/>
-The line below this paragraph, {{ABSTRACT_PUBMED_22118466}}, adds the Publication Abstract to the page
+__TOC__
-(as it appears on PubMed at http://www.pubmed.gov), where 22118466 is the PubMed ID number.
+</StructureSection>
--->
-{{ABSTRACT_PUBMED_22118466}}
-==About this Structure==
-[[3zyl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZYL OCA].
-==Reference==
-<ref group="xtra">PMID:022118466</ref><references group="xtra"/>
 [[Category: Rattus norvegicus]]
 [[Category: Graham, S C.]]