2cjm: Difference between revisions

Line 4:

==Overview==

Inhibition of cyclin-dependent kinase 1 (CDK1) activity by Tyr-15, phosphorylation directly regulates entry into mitosis and is an important, element in the control of the unperturbed cell cycle. Active site, phosphorylation of other members of the CDK family that regulate cell, cycle progression instates checkpoints that are fundamental to eukaryotic, cell cycle regulation. Kinetic and crystallographic analyses of, CDK2-cyclin A complexes reveal that this inhibitory mechanism operates, through steric blockade of peptide substrate binding and through the, creation of an environment that favors a non-productive conformation of, the terminal group of ATP. By contrast, tyrosine phosphorylation of CDK2, alters neither its Km for ATP nor its significant intrinsic ATPase, activity. Tyr-15-phosphorylated CDK2 retains trace protein phosphorylation, activity that should be considered in quantitative and qualitative cell, cycle models.

Inhibition of cyclin-dependent kinase 1 (CDK1) activity by Tyr-15 phosphorylation directly regulates entry into mitosis and is an important element in the control of the unperturbed cell cycle. Active site phosphorylation of other members of the CDK family that regulate cell cycle progression instates checkpoints that are fundamental to eukaryotic cell cycle regulation. Kinetic and crystallographic analyses of CDK2-cyclin A complexes reveal that this inhibitory mechanism operates through steric blockade of peptide substrate binding and through the creation of an environment that favors a non-productive conformation of the terminal group of ATP. By contrast, tyrosine phosphorylation of CDK2 alters neither its Km for ATP nor its significant intrinsic ATPase activity. Tyr-15-phosphorylated CDK2 retains trace protein phosphorylation activity that should be considered in quantitative and qualitative cell cycle models.

==About this Structure==

Line 13:

[[Category: Homo sapiens]]

[[Category: Protein complex]]

[[Category: Transferred entry: 2.7.11.1]]

[[Category: Transferred entry: 2 7.11 1]]

[[Category: Endicott, J.A.]]

[[Category: Endicott, J A.]]

[[Category: Johnson, T.]]

[[Category: Lindert, L.]]

[[Category: Noble, M.E.M.]]

[[Category: Noble, M E.M.]]

[[Category: Tucker, J.]]

[[Category: Welburn, J.P.I.]]

[[Category: Welburn, J P.I.]]

[[Category: Willis, A.]]

[[Category: ATP]]

Line 39:

[[Category: transferase]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun~~ Feb ~~3 10~~:36:09 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:49:25 2008''

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 4: / Line 4: @@
 ==Overview==
-Inhibition of cyclin-dependent kinase 1 (CDK1) activity by Tyr-15, phosphorylation directly regulates entry into mitosis and is an important, element in the control of the unperturbed cell cycle. Active site, phosphorylation of other members of the CDK family that regulate cell, cycle progression instates checkpoints that are fundamental to eukaryotic, cell cycle regulation. Kinetic and crystallographic analyses of, CDK2-cyclin A complexes reveal that this inhibitory mechanism operates, through steric blockade of peptide substrate binding and through the, creation of an environment that favors a non-productive conformation of, the terminal group of ATP. By contrast, tyrosine phosphorylation of CDK2, alters neither its Km for ATP nor its significant intrinsic ATPase, activity. Tyr-15-phosphorylated CDK2 retains trace protein phosphorylation, activity that should be considered in quantitative and qualitative cell, cycle models.
+Inhibition of cyclin-dependent kinase 1 (CDK1) activity by Tyr-15 phosphorylation directly regulates entry into mitosis and is an important element in the control of the unperturbed cell cycle. Active site phosphorylation of other members of the CDK family that regulate cell cycle progression instates checkpoints that are fundamental to eukaryotic cell cycle regulation. Kinetic and crystallographic analyses of CDK2-cyclin A complexes reveal that this inhibitory mechanism operates through steric blockade of peptide substrate binding and through the creation of an environment that favors a non-productive conformation of the terminal group of ATP. By contrast, tyrosine phosphorylation of CDK2 alters neither its Km for ATP nor its significant intrinsic ATPase activity. Tyr-15-phosphorylated CDK2 retains trace protein phosphorylation activity that should be considered in quantitative and qualitative cell cycle models.
 ==About this Structure==
@@ Line 13: / Line 13: @@
 [[Category: Homo sapiens]]
 [[Category: Protein complex]]
-[[Category: Transferred entry: 2.7.11.1]]
+[[Category: Transferred entry: 2 7.11 1]]
-[[Category: Endicott, J.A.]]
+[[Category: Endicott, J A.]]
 [[Category: Johnson, T.]]
 [[Category: Lindert, L.]]
-[[Category: Noble, M.E.M.]]
+[[Category: Noble, M E.M.]]
 [[Category: Tucker, J.]]
-[[Category: Welburn, J.P.I.]]
+[[Category: Welburn, J P.I.]]
 [[Category: Willis, A.]]
 [[Category: ATP]]
@@ Line 39: / Line 39: @@
 [[Category: transferase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb  3 10:36:09 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:49:25 2008''