Sandbox 666: Difference between revisions
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The reaction is due to a catalytic sequence motif which is found in most type II restriction endonucleases: the PD…D/EXK motif. For EcoRI, this catalytic sequence is PD91 …E111AK. This motif is also responsible for Mg2+ binding(Asp90 and Glu111). | The reaction is due to a catalytic sequence motif which is found in most type II restriction endonucleases: the PD…D/EXK motif. For EcoRI, this catalytic sequence is PD91 …E111AK. This motif is also responsible for Mg2+ binding(Asp90 and Glu111). | ||
== Application of EcoRI in molecular biology == | == Application of EcoRI in molecular biology == | ||
Type II restriction endonuclease like EcoRI are often used in molecular biology for their capacity to cut precisely DNA on specific restriction site. That makes them useful tools for gene cloning. By using two different restriction enzymes, it is possible to do directional cloning which is very important if you want to insert a gene in an expression vector. | Type II restriction endonuclease like EcoRI are often used in molecular biology for their capacity to cut precisely DNA on specific restriction site. That makes them useful tools for gene cloning. By using two different restriction enzymes, it is possible to do directional cloning which is very important if you want to insert a gene in an expression vector. | ||
== References == | |||
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Please use the "3D" button above this box to insert a Jmol applet (molecule) on this page. | Please use the "3D" button above this box to insert a Jmol applet (molecule) on this page. | ||
