Sand box 211: Difference between revisions
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== '''Applications''' == | == '''Applications''' == | ||
The T5 5’ exonuclease is used for plasmid and oligonucleotide mutagenesis. It can be used to destroy anything apart from pure closed circular plasmid, so it is useful to increase transfection efficiency and to reduce background in cloning experiments. T5 5’exonuclease is also used to remove denatured DNA from alkaline-based plasmid purification for improved cloning procedures. | |||
== '''References''' == | == '''References''' == | ||
