Sand box 211: Difference between revisions
From Proteopedia
Jump to navigationJump to search
Celina Pinto (talk | contribs) |
Celina Pinto (talk | contribs) |
||
| Line 28: | Line 28: | ||
<Structure load='1EXN' size='500' frame='true' align='right' caption='T5 5'exonuclease' scene='Insert optional scene name here' /> | <Structure load='1EXN' size='500' frame='true' align='right' caption='T5 5'exonuclease' scene='Insert optional scene name here' /> | ||
T5 5'- | <scene name='Sand_box_211/1exn/1'>T5 5'-exonucleaseT5</scene> is a homodimeric protein composed of two identical chains, <scene name='Sand_box_211/Vghjvjh/2'>chain a</scene> and <scene name='Sand_box_211/Vhj/1'>chain b</scene>. | ||
Both chains contain a hole, bound by a helical arch composed of two helices in which hydrophobic and positively charged residues are located. The helical arch is situated in front of the active site and only single-stranded DNA can pass through it. Since the enzyme is able to cleave double-stranded DNA, the enzyme has a conformational flexibility to facilitate DNA threading which is required to process the 5' nuclease substrates in the active site. | Both chains contain a hole, bound by a helical arch composed of two helices in which hydrophobic and positively charged residues are located. The helical arch is situated in front of the active site and only single-stranded DNA can pass through it. Since the enzyme is able to cleave double-stranded DNA, the enzyme has a conformational flexibility to facilitate DNA threading which is required to process the 5' nuclease substrates in the active site. | ||
