3t79: Difference between revisions

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[[~~Image~~:~~3t79~~.~~png~~|~~left~~|~~200px~~]]

==Ndc10: a platform for inner kinetochore assembly in budding yeast==

== Structural highlights ==

<table><tr><td colspan='2'>[[3t79]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Kluyveromyces_lactis Kluyveromyces lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T79 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T79 FirstGlance]. <br>

</td></tr><tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3sqi|3sqi]]</td></tr>

<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">KLLA0E03807g ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28985 Kluyveromyces lactis])</td></tr>

<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t79 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t79 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3t79 RCSB], [http://www.ebi.ac.uk/pdbsum/3t79 PDBsum]</span></td></tr>

<table>

== Publication Abstract from PubMed ==

Kinetochores link centromeric DNA to spindle microtubules and ensure faithful chromosome segregation during mitosis. In point-centromere yeasts, the CBF3 complex Skp1-Ctf13-(Cep3)(2)-(Ndc10)(2) recognizes a conserved centromeric DNA element through contacts made by Cep3 and Ndc10. We describe here the five-domain organization of Kluyveromyces lactis Ndc10 and the structure at 2.8 A resolution of domains I-II (residues 1-402) bound to DNA. The structure resembles tyrosine DNA recombinases, although it lacks both endonuclease and ligase activities. Structural and biochemical data demonstrate that each subunit of the Ndc10 dimer binds a separate fragment of DNA, suggesting that Ndc10 stabilizes a DNA loop at the centromere. We describe in vitro association experiments showing that specific domains of Ndc10 interact with each of the known inner-kinetochore proteins or protein complexes in budding yeast. We propose that Ndc10 provides a central platform for inner-kinetochore assembly.

~~<!--~~

Ndc10 is a platform for inner kinetochore assembly in budding yeast.,Cho US, Harrison SC Nat Struct Mol Biol. 2011 Dec 4. doi: 10.1038/nsmb.2178. PMID:22139014<ref>PMID:22139014</ref>

~~The line below this paragraph~~, ~~containing "STRUCTURE_3t79"~~, ~~creates the "Structure Box" on the page~~.

~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~

~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~

~~or leave the SCENE parameter empty for the default display~~.

-->

~~{{STRUCTURE_3t79| PDB=3t79 | SCENE= }}~~

~~===Ndc10:~~ a ~~platform for inner kinetochore assembly in budding yeast===~~

From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>

</div>

== References ==

~~<!--~~

~~The line below this paragraph, {{ABSTRACT_PUBMED_22139014}}, adds~~ the ~~Publication Abstract to the page~~

__TOC__

~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 22139014 is the PubMed ID number~~.

</StructureSection>

-->

~~{{ABSTRACT_PUBMED_22139014}}~~

~~==About this Structure==~~

~~[[3t79]] is a 6 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Kluyveromyces_lactis Kluyveromyces lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T79 OCA].~~

==~~Reference~~==

<~~ref group="xtra">PMID:022139014<~~/~~ref~~><~~references group="xtra"~~/>

[[Category: Kluyveromyces lactis]]

[[Category: Cho, U S.]]

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@@ Line 1: / Line 1: @@
-[[Image:3t79.png|left|200px]]
+==Ndc10: a platform for inner kinetochore assembly in budding yeast==
+<StructureSection load='3t79' size='340' side='right' caption='[[3t79]], [[Resolution|resolution]] 3.61&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[3t79]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Kluyveromyces_lactis Kluyveromyces lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T79 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T79 FirstGlance]. <br>
+</td></tr><tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3sqi|3sqi]]</td></tr>
+<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">KLLA0E03807g ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28985 Kluyveromyces lactis])</td></tr>
+<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t79 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t79 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3t79 RCSB], [http://www.ebi.ac.uk/pdbsum/3t79 PDBsum]</span></td></tr>
+<table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Kinetochores link centromeric DNA to spindle microtubules and ensure faithful chromosome segregation during mitosis. In point-centromere yeasts, the CBF3 complex Skp1-Ctf13-(Cep3)(2)-(Ndc10)(2) recognizes a conserved centromeric DNA element through contacts made by Cep3 and Ndc10. We describe here the five-domain organization of Kluyveromyces lactis Ndc10 and the structure at 2.8 A resolution of domains I-II (residues 1-402) bound to DNA. The structure resembles tyrosine DNA recombinases, although it lacks both endonuclease and ligase activities. Structural and biochemical data demonstrate that each subunit of the Ndc10 dimer binds a separate fragment of DNA, suggesting that Ndc10 stabilizes a DNA loop at the centromere. We describe in vitro association experiments showing that specific domains of Ndc10 interact with each of the known inner-kinetochore proteins or protein complexes in budding yeast. We propose that Ndc10 provides a central platform for inner-kinetochore assembly.
-<!--
+Ndc10 is a platform for inner kinetochore assembly in budding yeast.,Cho US, Harrison SC Nat Struct Mol Biol. 2011 Dec 4. doi: 10.1038/nsmb.2178. PMID:22139014<ref>PMID:22139014</ref>
-The line below this paragraph, containing "STRUCTURE_3t79", creates the "Structure Box" on the page.
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-or leave the SCENE parameter empty for the default display.
--->
-{{STRUCTURE_3t79|  PDB=3t79  |  SCENE=  }}
-===Ndc10: a platform for inner kinetochore assembly in budding yeast===
+From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
+</div>
+== References ==
-<!--
+<references/>
-The line below this paragraph, {{ABSTRACT_PUBMED_22139014}}, adds the Publication Abstract to the page
+__TOC__
-(as it appears on PubMed at http://www.pubmed.gov), where 22139014 is the PubMed ID number.
+</StructureSection>
--->
-{{ABSTRACT_PUBMED_22139014}}
-==About this Structure==
-[[3t79]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Kluyveromyces_lactis Kluyveromyces lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T79 OCA].
-==Reference==
-<ref group="xtra">PMID:022139014</ref><references group="xtra"/>
 [[Category: Kluyveromyces lactis]]
 [[Category: Cho, U S.]]