2j0s: Difference between revisions

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==Overview==

The exon junction complex (EJC) plays a major role in posttranscriptional, regulation of mRNA in metazoa. The EJC is deposited onto mRNA during, splicing and is transported to the cytoplasm where it influences, translation, surveillance, and localization of the spliced mRNA. The, complex is formed by the association of four proteins (eIF4AIII, Barentsz, [Btz], Mago, and Y14), mRNA, and ATP. The 2.2 A resolution structure of, the EJC reveals how it stably locks onto mRNA. The DEAD-box protein, eIF4AIII encloses an ATP molecule and provides the binding sites for six, ribonucleotides. Btz wraps around eIF4AIII and stacks against the 5', nucleotide. An intertwined network of interactions anchors Mago-Y14 and, Btz at the interface between the two domains of eIF4AIII, effectively, stabilizing the ATP bound state. Comparison with the structure of the, eIF4AIII-Btz subcomplex that we have also determined reveals that large, conformational changes are required upon EJC assembly and disassembly.

The exon junction complex (EJC) plays a major role in posttranscriptional regulation of mRNA in metazoa. The EJC is deposited onto mRNA during splicing and is transported to the cytoplasm where it influences translation, surveillance, and localization of the spliced mRNA. The complex is formed by the association of four proteins (eIF4AIII, Barentsz [Btz], Mago, and Y14), mRNA, and ATP. The 2.2 A resolution structure of the EJC reveals how it stably locks onto mRNA. The DEAD-box protein eIF4AIII encloses an ATP molecule and provides the binding sites for six ribonucleotides. Btz wraps around eIF4AIII and stacks against the 5' nucleotide. An intertwined network of interactions anchors Mago-Y14 and Btz at the interface between the two domains of eIF4AIII, effectively stabilizing the ATP bound state. Comparison with the structure of the eIF4AIII-Btz subcomplex that we have also determined reveals that large conformational changes are required upon EJC assembly and disassembly.

==About this Structure==

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[[Category: transport]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:58:06 2008''

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@@ Line 4: / Line 4: @@
 ==Overview==
-The exon junction complex (EJC) plays a major role in posttranscriptional, regulation of mRNA in metazoa. The EJC is deposited onto mRNA during, splicing and is transported to the cytoplasm where it influences, translation, surveillance, and localization of the spliced mRNA. The, complex is formed by the association of four proteins (eIF4AIII, Barentsz, [Btz], Mago, and Y14), mRNA, and ATP. The 2.2 A resolution structure of, the EJC reveals how it stably locks onto mRNA. The DEAD-box protein, eIF4AIII encloses an ATP molecule and provides the binding sites for six, ribonucleotides. Btz wraps around eIF4AIII and stacks against the 5', nucleotide. An intertwined network of interactions anchors Mago-Y14 and, Btz at the interface between the two domains of eIF4AIII, effectively, stabilizing the ATP bound state. Comparison with the structure of the, eIF4AIII-Btz subcomplex that we have also determined reveals that large, conformational changes are required upon EJC assembly and disassembly.
+The exon junction complex (EJC) plays a major role in posttranscriptional regulation of mRNA in metazoa. The EJC is deposited onto mRNA during splicing and is transported to the cytoplasm where it influences translation, surveillance, and localization of the spliced mRNA. The complex is formed by the association of four proteins (eIF4AIII, Barentsz [Btz], Mago, and Y14), mRNA, and ATP. The 2.2 A resolution structure of the EJC reveals how it stably locks onto mRNA. The DEAD-box protein eIF4AIII encloses an ATP molecule and provides the binding sites for six ribonucleotides. Btz wraps around eIF4AIII and stacks against the 5' nucleotide. An intertwined network of interactions anchors Mago-Y14 and Btz at the interface between the two domains of eIF4AIII, effectively stabilizing the ATP bound state. Comparison with the structure of the eIF4AIII-Btz subcomplex that we have also determined reveals that large conformational changes are required upon EJC assembly and disassembly.
 ==About this Structure==
@@ Line 39: / Line 39: @@
 [[Category: transport]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb  3 10:40:20 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:58:06 2008''