Sandbox 666: Difference between revisions
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The reaction is due to a catalytic sequence motif which is found in most type II restriction endonucleases: the PD…D/EXK motif. For EcoRI,<scene name='Sandbox_666/Catalytic_core/3'>this catalytic sequence</scene> is PD<sup>91</sup> …E<sup>111</sup>AK. This motif is also responsible for Mg2+ binding(Asp90 and Glu111). [[Image:Catalytic 1ERI.png | thumb | The catalytic core of Eco RI with the target DNA|left| | The reaction is due to a catalytic sequence motif which is found in most type II restriction endonucleases: the PD…D/EXK motif. For EcoRI,<scene name='Sandbox_666/Catalytic_core/3'>this catalytic sequence</scene> is PD<sup>91</sup> …E<sup>111</sup>AK. This motif is also responsible for Mg2+ binding(Asp90 and Glu111). | ||
[[Image:Catalytic 1ERI.png | thumb | The catalytic core of Eco RI with the target DNA|left|400px]]<ref> | |||
Structure and function of type II restriction endonucleases | Structure and function of type II restriction endonucleases | ||
Alfred Pingoud, Albert Jeltsch | Alfred Pingoud, Albert Jeltsch | ||
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Type II restriction endonuclease like EcoRI are often used in molecular biology for their capacity to cut precisely DNA on specific restriction site. That makes them useful tools for gene cloning. By using two different restriction enzymes, it is possible to do directional cloning which is very important if you want to insert a gene in an expression vector. | Type II restriction endonuclease like EcoRI are often used in molecular biology for their capacity to cut precisely DNA on specific restriction site. That makes them useful tools for gene cloning. By using two different restriction enzymes, it is possible to do directional cloning which is very important if you want to insert a gene in an expression vector. | ||
== Links == | == Links == | ||
