Sandbox 666: Difference between revisions
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During the formation of the specific complex, the majority of the water molecules and ions are released and more and more direct interactions between the enzyme and the DNA are formed: some water molecules are immobilized between the enzyme and the DNA and formed hydrogen bounds, whose are important for the recognition and catalysis. Metal divalent ions, Mg2+, play also an essential role in the recognition process. The two homodimers of'' Eco''RI cooperate in the binding and cleavage of their substrate and are symmetrically bound to the DNA. The specific complex formation leads to conformational changes of the protein and the DNA, thus catalytic centers of the both subunits are activated and the both DNA strands are simultaneously cleaved. | During the formation of the specific complex, the majority of the water molecules and ions are released and more and more direct interactions between the enzyme and the DNA are formed: some water molecules are immobilized between the enzyme and the DNA and formed hydrogen bounds, whose are important for the recognition and catalysis. Metal divalent ions, Mg2+, play also an essential role in the recognition process. The two homodimers of'' Eco''RI cooperate in the binding and cleavage of their substrate and are symmetrically bound to the DNA. The specific complex formation leads to conformational changes of the protein and the DNA, thus catalytic centers of the both subunits are activated and the both DNA strands are simultaneously cleaved. | ||
Specific binding bend the DNA by about 12° and involve interactions between | Specific binding bend the DNA by about 12° and involve interactions between ''Eco''RI and DNA bases and phosphodiester backbones over approximately 10-12 bp. Moreover the residues involve to binding could participate at the catalysis. The number of interactions between ''Eco''RI and the recognition sequence is maximal. | ||
The restriction enzymes are highly specific and highly cooperative. | The restriction enzymes are highly specific and highly cooperative. | ||
