Sandbox 206: Difference between revisions
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=== Introduction=== | === Introduction=== | ||
Yellow Fluorescent protein (YFP) is a variant of Green fluorescent protein (GFP). This protein is very useful like a biomarker in a lot of biological applications. First, the GFP was mutated In YFP. But the YFP had a low fluorescence because of a slow maturation and it was very sensitive to experimental condition (pH, Cl-). To increase the brightness of the protein, maturation speed and other properties, five mutations were done to obtain Venus, a Yellow Fluorescent Protein less sensitive to experimental condition. <ref> | Yellow Fluorescent protein (YFP) is a variant of Green fluorescent protein (GFP). This protein is very useful like a biomarker in a lot of biological applications. First, the GFP was mutated In YFP. But the YFP had a low fluorescence because of a slow maturation and it was very sensitive to experimental condition (pH, Cl-). To increase the brightness of the protein, maturation speed and other properties, five mutations were done to obtain Venus, a Yellow Fluorescent Protein less sensitive to experimental condition. <ref> Crystal Structure of Venus, a Yellow Fluorescent Protein with Improved Maturation and Reduced Environmental Sensitivity Rekas, A., Alattia, J.R., Nagai, T., Miyawaki, A., and Ikura M., 2002 </ref> | ||
=== Presentation of the molecule === | === Presentation of the molecule === | ||
