Sandbox 206: Difference between revisions

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=== Introduction===

Yellow Fluorescent protein (YFP) is a variant of Green fluorescent protein (GFP). This protein is very useful like a biomarker in a lot of biological applications. First, the GFP was mutated In YFP. But the YFP had a low fluorescence because of a slow maturation and it was very sensitive to experimental condition (pH, Cl-). To increase the brightness of the protein, maturation speed and other properties, five mutations were done to obtain Venus, a Yellow Fluorescent Protein less sensitive to experimental condition. <ref> Crystal Structure of Venus, a Yellow Fluorescent Protein with Improved Maturation and Reduced Environmental Sensitivity Rekas, A., Alattia, J.R., Nagai, T., Miyawaki, A., and Ikura M., 2002 </ref>

Yellow Fluorescent protein (YFP) is a variant of Green fluorescent protein (GFP). This protein is very useful like a biomarker in a lot of biological applications. First, the GFP was mutated In YFP. But the YFP had a low fluorescence because of a slow maturation and it was very sensitive to experimental condition (pH, Cl-). To increase the brightness of the protein, maturation speed and other properties, five mutations were done to obtain Venus, a Yellow Fluorescent Protein less sensitive to experimental condition. <ref> Crystal Structure of Venus, a Yellow Fluorescent Protein with Improved Maturation and Reduced Environmental Sensitivity Rekas, A., Alattia, J.R., Nagai, T., Miyawaki, A., and Ikura M., 2002 Pubmed : [http://www.ncbi.nlm.nih.gov/pubmed/12370172?dopt=Abstract 12370172] DOI : [http://www.jbc.org/content/277/52/50573 10.1074/jbc.M209524200]</ref>

=== Presentation of the molecule ===

Sandbox 206: Difference between revisions

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 === Introduction===
-Yellow Fluorescent protein (YFP) is a variant of Green fluorescent protein (GFP). This protein is very useful like a biomarker in a lot of biological applications. First, the GFP was mutated In YFP. But the YFP had a low fluorescence because of a slow maturation and it was very sensitive to experimental condition (pH, Cl-). To increase the brightness of the protein, maturation speed and other properties, five mutations were done to obtain Venus, a Yellow Fluorescent Protein less sensitive to experimental condition. <ref> Crystal Structure of Venus, a Yellow Fluorescent Protein with Improved Maturation and Reduced Environmental Sensitivity Rekas, A., Alattia, J.R., Nagai, T., Miyawaki, A., and Ikura M., 2002 </ref>
+Yellow Fluorescent protein (YFP) is a variant of Green fluorescent protein (GFP). This protein is very useful like a biomarker in a lot of biological applications. First, the GFP was mutated In YFP. But the YFP had a low fluorescence because of a slow maturation and it was very sensitive to experimental condition (pH, Cl-). To increase the brightness of the protein, maturation speed and other properties, five mutations were done to obtain Venus, a Yellow Fluorescent Protein less sensitive to experimental condition. <ref> Crystal Structure of Venus, a Yellow Fluorescent Protein with Improved Maturation and Reduced Environmental Sensitivity Rekas, A., Alattia, J.R., Nagai, T., Miyawaki, A., and Ikura M., 2002 Pubmed : [http://www.ncbi.nlm.nih.gov/pubmed/12370172?dopt=Abstract 12370172] DOI : [http://www.jbc.org/content/277/52/50573 10.1074/jbc.M209524200]</ref>
 === Presentation of the molecule ===