3qac: Difference between revisions

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[[~~Image~~:~~3qac~~.~~png~~|~~left~~|~~200px~~]]

==Structure of amaranth 11S proglobulin seed storage protein from Amaranthus hypochondriacus L.==

== Structural highlights ==

<table><tr><td colspan='2'>[[3qac]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Amaranthus_hypochondriacus Amaranthus hypochondriacus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QAC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QAC FirstGlance]. <br>

<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qac FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qac OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qac RCSB], [http://www.ebi.ac.uk/pdbsum/3qac PDBsum]</span></td></tr>

<table>

== Publication Abstract from PubMed ==

11S globulin is one of the major seed storage proteins in amaranth. Recombinant protein was produced as up to approximately 80% of the total bacterial protein using Escherichia coli Rosetta-gami (DE3) containing pET21d with amaranth 11S globulin cDNA. The best expression condition was at 302 K for 20 h using LB medium containing 0.5 M NaCl. The recombinant protein was easily separated from most of the Escherichia coli proteins by precipitation with 0-40% ammonium sulfate solution. It formed aggregates at low temperature and at low salt concentrations. This behaviour may imply that it has a more hydrophobic nature than other 11S seed globulins. The crystals diffracted to 6 A resolution and belonged to space group P6(3), with unit-cell parameters a=b=97.6, c=74.8 A, gamma=120.0 degrees. One subunit of a trimer was estimated to be present in the asymmetric unit, assuming a Vsol of 41%. To obtain the complete structure solution, experiments to improve crystallization and flash-cooling conditions are in progress.

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Expression, purification and preliminary crystallization of amaranth 11S proglobulin seed storage protein from Amaranthus hypochondriacus L.,Tandang-Silvas MR, Carrazco-Pena L, Barba de la Rosa AP, Osuna-Castro JA, Utsumi S, Mikami B, Maruyama N Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Aug 1;66(Pt, 8):919-22. Epub 2010 Jul 29. PMID:20693668<ref>PMID:20693668</ref>

~~The line below this paragraph~~, ~~containing "STRUCTURE_3qac"~~, ~~creates the "Structure Box" on the page~~.

~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)

~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~

~~or leave the SCENE parameter empty for the default display~~.

-->

~~{{STRUCTURE_3qac| PDB=3qac | SCENE= }}~~

~~===Structure~~ of ~~amaranth 11S proglobulin seed storage protein from Amaranthus hypochondriacus L.===~~

From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>

</div>

== References ==

~~<!--~~

~~The line below this paragraph, {{ABSTRACT_PUBMED_20693668}}, adds the Publication Abstract to~~ the ~~page~~

__TOC__

~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 20693668 is the PubMed ID number~~.

</StructureSection>

-->

~~{{ABSTRACT_PUBMED_20693668}}~~

~~==About this Structure==~~

~~[[3qac]] is a 1 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Amaranthus_hypochondriacus Amaranthus hypochondriacus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QAC OCA].~~

==~~Reference~~==

<~~ref group="xtra">PMID:020693668<~~/~~ref~~><~~references group="xtra"~~/>

[[Category: Amaranthus hypochondriacus]]

[[Category: Carrazco-Pena, L.]]

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-[[Image:3qac.png|left|200px]]
+==Structure of amaranth 11S proglobulin seed storage protein from Amaranthus hypochondriacus L.==
+<StructureSection load='3qac' size='340' side='right' caption='[[3qac]], [[Resolution|resolution]] 2.27&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[3qac]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Amaranthus_hypochondriacus Amaranthus hypochondriacus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QAC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QAC FirstGlance]. <br>
+</td></tr><tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3kgl|3kgl]], [[3ksc|3ksc]], [[2e9q|2e9q]], [[2d5f|2d5f]], [[2d5h|2d5h]], [[1od5|1od5]], [[1fxz|1fxz]]</td></tr>
+<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qac FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qac OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qac RCSB], [http://www.ebi.ac.uk/pdbsum/3qac PDBsum]</span></td></tr>
+<table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+S globulin is one of the major seed storage proteins in amaranth. Recombinant protein was produced as up to approximately 80% of the total bacterial protein using Escherichia coli Rosetta-gami (DE3) containing pET21d with amaranth 11S globulin cDNA. The best expression condition was at 302 K for 20 h using LB medium containing 0.5 M NaCl. The recombinant protein was easily separated from most of the Escherichia coli proteins by precipitation with 0-40% ammonium sulfate solution. It formed aggregates at low temperature and at low salt concentrations. This behaviour may imply that it has a more hydrophobic nature than other 11S seed globulins. The crystals diffracted to 6 A resolution and belonged to space group P6(3), with unit-cell parameters a=b=97.6, c=74.8 A, gamma=120.0 degrees. One subunit of a trimer was estimated to be present in the asymmetric unit, assuming a Vsol of 41%. To obtain the complete structure solution, experiments to improve crystallization and flash-cooling conditions are in progress.
-<!--
+Expression, purification and preliminary crystallization of amaranth 11S proglobulin seed storage protein from Amaranthus hypochondriacus L.,Tandang-Silvas MR, Carrazco-Pena L, Barba de la Rosa AP, Osuna-Castro JA, Utsumi S, Mikami B, Maruyama N Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Aug 1;66(Pt, 8):919-22. Epub 2010 Jul 29. PMID:20693668<ref>PMID:20693668</ref>
-The line below this paragraph, containing "STRUCTURE_3qac", creates the "Structure Box" on the page.
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-or leave the SCENE parameter empty for the default display.
--->
-{{STRUCTURE_3qac|  PDB=3qac  |  SCENE=  }}
-===Structure of amaranth 11S proglobulin seed storage protein from Amaranthus hypochondriacus L.===
+From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
+</div>
+== References ==
-<!--
+<references/>
-The line below this paragraph, {{ABSTRACT_PUBMED_20693668}}, adds the Publication Abstract to the page
+__TOC__
-(as it appears on PubMed at http://www.pubmed.gov), where 20693668 is the PubMed ID number.
+</StructureSection>
--->
-{{ABSTRACT_PUBMED_20693668}}
-==About this Structure==
-[[3qac]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Amaranthus_hypochondriacus Amaranthus hypochondriacus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QAC OCA].
-==Reference==
-<ref group="xtra">PMID:020693668</ref><references group="xtra"/>
 [[Category: Amaranthus hypochondriacus]]
 [[Category: Carrazco-Pena, L.]]