2v1u: Difference between revisions

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==Overview==

DNA replication in archaea and in eukaryotes share many similarities. We, report the structure of an archaeal origin recognition complex protein, ORC1, bound to an origin recognition box, a DNA sequence that is found in, multiple copies at replication origins. DNA binding is mediated, principally by a C-terminal winged helix domain that inserts deeply into, the major and minor grooves, widening them both. However, additional DNA, contacts are made with the N-terminal AAA+ domain, which inserts into the, minor groove at a characteristic G-rich sequence, inducing a 35 degrees, bend in the duplex and providing directionality to the binding site. Both, contact regions also induce substantial unwinding of the DNA. The, structure provides insight into the initial step in assembly of a, replication origin and recruitment of minichromosome maintenance (MCM), helicase to that origin.

DNA replication in archaea and in eukaryotes share many similarities. We report the structure of an archaeal origin recognition complex protein, ORC1, bound to an origin recognition box, a DNA sequence that is found in multiple copies at replication origins. DNA binding is mediated principally by a C-terminal winged helix domain that inserts deeply into the major and minor grooves, widening them both. However, additional DNA contacts are made with the N-terminal AAA+ domain, which inserts into the minor groove at a characteristic G-rich sequence, inducing a 35 degrees bend in the duplex and providing directionality to the binding site. Both contact regions also induce substantial unwinding of the DNA. The structure provides insight into the initial step in assembly of a replication origin and recruitment of minichromosome maintenance (MCM) helicase to that origin.

==About this Structure==

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[[Category: Protein complex]]

[[Category: Gaudier, M.]]

[[Category: Schuwirth, B.S.]]

[[Category: Schuwirth, B S.]]

[[Category: Westcott, S.L.]]

[[Category: Westcott, S L.]]

[[Category: Wigley, D.B.]]

[[Category: Wigley, D B.]]

[[Category: ADP]]

[[Category: MG]]

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[[Category: replication]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun~~ Feb ~~3 10~~:49:20 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:52:31 2008''

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@@ Line 4: / Line 4: @@
 ==Overview==
-DNA replication in archaea and in eukaryotes share many similarities. We, report the structure of an archaeal origin recognition complex protein, ORC1, bound to an origin recognition box, a DNA sequence that is found in, multiple copies at replication origins. DNA binding is mediated, principally by a C-terminal winged helix domain that inserts deeply into, the major and minor grooves, widening them both. However, additional DNA, contacts are made with the N-terminal AAA+ domain, which inserts into the, minor groove at a characteristic G-rich sequence, inducing a 35 degrees, bend in the duplex and providing directionality to the binding site. Both, contact regions also induce substantial unwinding of the DNA. The, structure provides insight into the initial step in assembly of a, replication origin and recruitment of minichromosome maintenance (MCM), helicase to that origin.
+DNA replication in archaea and in eukaryotes share many similarities. We report the structure of an archaeal origin recognition complex protein, ORC1, bound to an origin recognition box, a DNA sequence that is found in multiple copies at replication origins. DNA binding is mediated principally by a C-terminal winged helix domain that inserts deeply into the major and minor grooves, widening them both. However, additional DNA contacts are made with the N-terminal AAA+ domain, which inserts into the minor groove at a characteristic G-rich sequence, inducing a 35 degrees bend in the duplex and providing directionality to the binding site. Both contact regions also induce substantial unwinding of the DNA. The structure provides insight into the initial step in assembly of a replication origin and recruitment of minichromosome maintenance (MCM) helicase to that origin.
 ==About this Structure==
@@ Line 14: / Line 14: @@
 [[Category: Protein complex]]
 [[Category: Gaudier, M.]]
-[[Category: Schuwirth, B.S.]]
+[[Category: Schuwirth, B S.]]
-[[Category: Westcott, S.L.]]
+[[Category: Westcott, S L.]]
-[[Category: Wigley, D.B.]]
+[[Category: Wigley, D B.]]
 [[Category: ADP]]
 [[Category: MG]]
@@ Line 27: / Line 27: @@
 [[Category: replication]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb  3 10:49:20 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:52:31 2008''