2li8: Difference between revisions

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[[Image:2li8.png|left|200px]]
==The solution structure of the Lin28-ZnF domains bound to AGGAGAU of pre-let-7 miRNA==
<StructureSection load='2li8' size='340' side='right' caption='[[2li8]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
== Structural highlights ==
[[2li8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LI8 OCA]. <br>
<b>Related:</b> [[2cqf|2cqf]]<br>
<b>Activity:</b> <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span><br>
== Publication Abstract from PubMed ==
Lin28 inhibits the biogenesis of let-7 miRNAs through a direct interaction with the terminal loop of pre-let-7. This interaction requires the zinc-knuckle domains of Lin28. We show that the zinc knuckle domains of Lin28 are sufficient to provide binding selectivity for pre-let-7 miRNAs and present the NMR structure of human Lin28 zinc knuckles bound to the short sequence 5'-AGGAGAU-3'. The structure reveals that each zinc knuckle recognizes an AG dinucleotide separated by a single nucleotide spacer. This defines a new 5'-NGNNG-3' consensus motif that explains how Lin28 selectively recognizes pre-let-7 family members. Binding assays in cell lysates and functional assays in cultured cells demonstrate that the interactions observed in the solution structure also occur between the full-length protein and members of the pre-let-7 family. The consensus sequence explains several seemingly disparate previously published observations on the binding properties of Lin28.


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Structural basis of pre-let-7 miRNA recognition by the zinc knuckles of pluripotency factor Lin28.,Loughlin FE, Gebert LF, Towbin H, Brunschweiger A, Hall J, Allain FH Nat Struct Mol Biol. 2011 Dec 11;19(1):84-9. doi: 10.1038/nsmb.2202. PMID:22157959<ref>PMID:22157959</ref>
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{{STRUCTURE_2li8|  PDB=2li8  |  SCENE=  }}


===The solution structure of the Lin28-ZnF domains bound to AGGAGAU of pre-let-7 miRNA===
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
 
== References ==
 
<references/>
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==About this Structure==
[[2li8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LI8 OCA].
 
==Reference==
<ref group="xtra">PMID:022157959</ref><references group="xtra"/>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Allain, F H.T.]]
[[Category: Allain, F H.T.]]
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