2opt: Difference between revisions

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[[Image:2opt.jpg|left|200px]]<br /><applet load="2opt" size="350" color="white" frame="true" align="right" spinBox="true"

caption="2opt" />

caption="2opt, resolution 2.05Å" />

''''''<br />

'''Crystal Structure of Apo ActR from Streptomyces coelicolor.'''<br />

==Overview==

Actinorhodin, an antibiotic produced by Streptomyces coelicolor, is, exported from the cell by the ActA efflux pump. actA is divergently, transcribed from actR, which encodes a TetR-like transcriptional, repressor. We showed previously that ActR represses transcription by, binding to an operator from the actA/actR intergenic region. Importantly, actinorhodin itself or various actinorhodin biosynthetic intermediates can, cause ActR to dissociate from its operator, leading to derepression. This, suggests that ActR may mediate timely self-resistance to an endogenously, produced antibiotic by responding to one of its biosynthetic precursors., Here, we report the structural basis for this precursor-mediated, derepression with crystal structures of homodimeric ActR by itself and in, complex with either actinorhodin or the actinorhodin biosynthetic, intermediate (S)-DNPA, [4-dihydro-9-hydroxy-1-methyl-10-oxo-3-H-naphtho-[2,3-c]-pyran-3-(S)-aceti, c acid]. The ligand-binding tunnel in each ActR monomer has a striking, hydrophilic/hydrophobic/hydrophilic arrangement of surface residues that, accommodate either one hexacyclic actinorhodin molecule or two, back-to-back tricyclic (S)-DNPA molecules. Moreover, our work also reveals, the strongest structural evidence to date that TetR-mediated antibiotic, resistance may have been acquired from an antibiotic-producer organism.

==About this Structure==

is a [http://en.wikipedia.org/wiki/~~Protein_complex Protein complex~~] structure of ~~sequences~~ from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA].

2OPT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OPT OCA].

[[Category: ~~Protein complex~~]]

==Reference==

Crystal Structures of the Streptomyces coelicolor TetR-Like Protein ActR Alone and in Complex with Actinorhodin or the Actinorhodin Biosynthetic Precursor (S)-DNPA., Willems AR, Tahlan K, Taguchi T, Zhang K, Lee ZZ, Ichinose K, Junop MS, Nodwell JR, J Mol Biol. 2008 Jan 4;. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18207163 18207163]

[[Category: Single protein]]

[[Category: Streptomyces coelicolor]]

[[Category: Junop, M.S.]]

[[Category: Willems, A.R.]]

[[Category: apo-protein]]

[[Category: helical protein]]

[[Category: tetr family]]

[[Category: transcriptional repressor]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 6 15:34:33 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 6 17:23:04 2008''

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@@ Line 1: / Line 1: @@
 [[Image:2opt.jpg|left|200px]]<br /><applet load="2opt" size="350" color="white" frame="true" align="right" spinBox="true"
-caption="2opt" />
+caption="2opt, resolution 2.05&Aring;" />
-''''''<br />
+'''Crystal Structure of Apo ActR from Streptomyces coelicolor.'''<br />
+==Overview==
+Actinorhodin, an antibiotic produced by Streptomyces coelicolor, is, exported from the cell by the ActA efflux pump. actA is divergently, transcribed from actR, which encodes a TetR-like transcriptional, repressor. We showed previously that ActR represses transcription by, binding to an operator from the actA/actR intergenic region. Importantly, actinorhodin itself or various actinorhodin biosynthetic intermediates can, cause ActR to dissociate from its operator, leading to derepression. This, suggests that ActR may mediate timely self-resistance to an endogenously, produced antibiotic by responding to one of its biosynthetic precursors., Here, we report the structural basis for this precursor-mediated, derepression with crystal structures of homodimeric ActR by itself and in, complex with either actinorhodin or the actinorhodin biosynthetic, intermediate (S)-DNPA, [4-dihydro-9-hydroxy-1-methyl-10-oxo-3-H-naphtho-[2,3-c]-pyran-3-(S)-aceti, c acid]. The ligand-binding tunnel in each ActR monomer has a striking, hydrophilic/hydrophobic/hydrophilic arrangement of surface residues that, accommodate either one hexacyclic actinorhodin molecule or two, back-to-back tricyclic (S)-DNPA molecules. Moreover, our work also reveals, the strongest structural evidence to date that TetR-mediated antibiotic, resistance may have been acquired from an antibiotic-producer organism.
 ==About this Structure==
- is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA].
+OPT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OPT OCA].
-[[Category: Protein complex]]
+==Reference==
+Crystal Structures of the Streptomyces coelicolor TetR-Like Protein ActR Alone and in Complex with Actinorhodin or the Actinorhodin Biosynthetic Precursor (S)-DNPA., Willems AR, Tahlan K, Taguchi T, Zhang K, Lee ZZ, Ichinose K, Junop MS, Nodwell JR, J Mol Biol. 2008 Jan 4;. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18207163 18207163]
+[[Category: Single protein]]
+[[Category: Streptomyces coelicolor]]
+[[Category: Junop, M.S.]]
+[[Category: Willems, A.R.]]
+[[Category: apo-protein]]
+[[Category: helical protein]]
+[[Category: tetr family]]
+[[Category: transcriptional repressor]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb  6 15:34:33 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb  6 17:23:04 2008''