2q2k: Difference between revisions
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New page: left|200px<br /><applet load="2q2k" size="350" color="white" frame="true" align="right" spinBox="true" caption="2q2k" /> ''''''<br /> ==About this Structure== is a [h... |
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[[Image:2q2k.jpg|left|200px]]<br /><applet load="2q2k" size="350" color="white" frame="true" align="right" spinBox="true" | [[Image:2q2k.jpg|left|200px]]<br /><applet load="2q2k" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="2q2k" /> | caption="2q2k, resolution 3.00Å" /> | ||
''''''<br /> | '''Structure of nucleic-acid binding protein'''<br /> | ||
==Overview== | |||
The stable inheritance of genetic material depends on accurate DNA, partition. Plasmids serve as tractable model systems to study DNA, segregation because they require only a DNA centromere, a, centromere-binding protein and a force-generating ATPase. The centromeres, of partition (par) systems typically consist of a tandem arrangement of, direct repeats. The best-characterized par system contains a, centromere-binding protein called ParR and an ATPase called ParM. In the, first step of segregation, multiple ParR proteins interact with the, centromere repeats to form a large nucleoprotein complex of unknown, structure called the segrosome, which binds ParM filaments. pSK41 ParR, binds a centromere consisting of multiple 20-base-pair (bp) tandem repeats, to mediate both transcription autoregulation and segregation. Here we, report the structure of the pSK41 segrosome revealed in the crystal, structure of a ParR-DNA complex. In the crystals, the 20-mer tandem, repeats stack pseudo-continuously to generate the full-length centromere, with the ribbon-helix-helix (RHH) fold of ParR binding successive DNA, repeats as dimer-of-dimers. Remarkably, the dimer-of-dimers assemble in a, continuous protein super-helical array, wrapping the DNA about its, positive convex surface to form a large segrosome with an open, solenoid-shaped structure, suggesting a mechanism for ParM capture and, subsequent plasmid segregation. | |||
==About this Structure== | ==About this Structure== | ||
2Q2K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus] with <scene name='pdbligand=EPE:'>EPE</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q2K OCA]. | |||
[[Category: | |||
==Reference== | |||
Segrosome structure revealed by a complex of ParR with centromere DNA., Schumacher MA, Glover TC, Brzoska AJ, Jensen SO, Dunham TD, Skurray RA, Firth N, Nature. 2007 Dec 20;450(7173):1268-71. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18097417 18097417] | |||
[[Category: Single protein]] | |||
[[Category: Staphylococcus aureus]] | |||
[[Category: Firth, N.]] | |||
[[Category: Glover, T.]] | |||
[[Category: Schumacher, M.A.]] | |||
[[Category: EPE]] | |||
[[Category: dna binding protein/dna complex]] | |||
[[Category: parb]] | |||
[[Category: partition]] | |||
[[Category: protein-dna]] | |||
[[Category: segregation]] | |||
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